XB-CLONE-4354121
Clone Name: IMAGE:7026540
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Gene:
ilf2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783187 [+]
taattaatccccccccccccccgtagtgtggctgtatcgagggccgagagggtgtccattgtgcgggagtgtgaggaacgaagatctgaaacatgagggg
tgaacgaggacgtggacgtggccgctttggctccagagtgggacctaacacaggtttcagacccttcgtccctcacattccctttgatttccacgtgtgt
gaaatggccttcccgcgtgtgaaacccgcccccgacgatagctcattcagcgagtcactgctcaaacgaaaccaagatctggcccccagcacagccgagc
aggcctcaatcctttctttggtcaccaaaatcaacaacgttatcgacaatttgatcgtagccccgggaaactttgaagtgcaaattgaggaggtgcgaca
ggtgggctccctacaagaaaggggaccatgagcaccgggccataatggttgcaagactttggccggtcattcccaaaaaaaatccctttcccccccaatt
tgggaaaaccccctttttcctggcgtttttgggggccattttaagagggggggggcccaaaaaacccccccctaaagggggagccccccggggggttatc
cccccccgctgaagaagtttttccccccccaaaagttgggggtagacaaaaagaaagggaaaaaatccggggggggtgttttattgtaaaaaataaaaag
ggcacacgattgaccagacagaaaatccacccccccccccgggggggggggggataaaaaaaagggccacccggtctttggcgacagccccacccgtttt
ttttgtttttccaccccgcccacccaatcttaactccttatgt
3' EST: CF783186 [+]tttttttttttttttctttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttggccaaaaaaaaaattt
ttggggggaaaaaaaaaaaaaaaaacaagggtccccccccgtaaaaaaaaagccccctggcaccctaaaaaaaaagggaaaaaaaacggggacccggggg
ttttggcccccaaaaaaaccccatggtttccgccccccccaagggggatccccaccccccgaaacctttaaaaaaaaatccccccgggggtcccagggcc
ccccccctttccccccccccgggggaaaccggggtttcaaccccccccccccccttccctgggggggggttccccaaggccctccctgaagggggcccaa
aaaccccccccccgggaaaaatttccgggggaaaaagggggggttcccccccccggcccaaaattttccggaaaccccccgggggaaaaaaacgggcccc
ccccggggggggggaaaaaaacatggccgggggcccccaaatcttggggggtgaaaccccaaaggggcccctttcccagggggcctgggggcccccctgc
cccgggagaaaaagggcggggggaaaactttttgggcccccttttgaaaaaaccttgaaggccccccccccccccgggggggggtttttcttggaggggc
taaggcccccaaagggcccaggatcccggggggatcaaagggtccaaatcccggaaaacccgcccccccaaggtttttttaaaaaccgggattaaaaccc
ttccctgcgggaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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