XB-CLONE-4354116
Clone Name: IMAGE:7026538
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Gene:
ocm4.4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783190 [+]
taattaatcccccccccccccggtgcaagcctcattgagaagatctgtaaagtcccgtgaaagaggaattaaggacacaaaatggccttaggtgctctcc
tgagtcaagctgacatctctgctgctctgcaggcttgccaagcccctgactccttcaactacaaatccttctttgcccagtctggcctgagcagcaagtc
cgtcgatgatgtgagaaacgcctttgccatccttgaccaggacaagagcggcttcattgaggaagatgaattgaaactgttcctccagaacttcagcgca
ggtgccagggctttgactgatgctgagaccaaggccttcctgaatgctggtgactctgatggtgatggcaaaattggagttgatgaattccaggctttgg
taaaggcttaaacattctaccacgtgagtctgtgaactttactaaacattaaccatccacaattttgactttttctaattaaactgaaataaacgggaat
ctggttttgtggaacacccccatctcttcgctgtgtttctatcccggggacaaatttttggaaacatcacctggcttggcgaatccaaagatttaattct
ggaaagaaaacattttcctttaccatcaacccaccatgggattcacctagtttctaaaaggggccgccccccccgggtggaaactttggccctgggcccc
aactttgttttattggcaccttaaaaatggggttacaaaaaaaaagcataagcatccccaaaattttcaccaaaaaaaggcatttttttttcccccggcc
attcccaattggtgggtttttgtgcccaacccccttcgaagggtatcccttatccaggggtccggggtaccggggaaatatanattttccgggccaacac
caacacgggggccagggaataaaacatcccggt
3' EST: CF783189 [+]ttgttggttgatggtaacgaaaatgtcttctttgcagaataaaatccttggattccgcaagcaggtgatgtttccaaaacttgtcccccggatagaaaca
cagcgaagagatggtggtgttccacaaaaccagatttccgtttatttcagtttaattagaaaaagtcaaaattgtggatggttaatgtttagtaaagttc
acagactcacgtggtagaatgtttaagcctttaccaaagcctggaattcatcaactccaattttgccatcaccatcagagtcaccagcattcaggaaggc
cttggtctcagcatcagtcaaagccctggcacctgcgctgaagttctggaggaacagtttcaattcatcttcctcaatgaagccgctcttgtcctggtca
aggatggcaaaggcgtttctcacatcatcgacggacttgctgctcaggccagactgggcaaagaaggatttgtagttgaaggagtcaggggcttggcaag
cctgcagagcagcagagatgtcagcttgactcaggagagcacctaaggccattttgtgtccttaattcctctttcacgggactttacagatcttctcaat
gaggctgccn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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