XB-CLONE-4354106
Clone Name: IMAGE:7026534
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Gene:
anapc5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080458 [+]
CGCTGTTGAGTGTATGGCAAAGTGGTGCCTGAGACGGAGCTTCTGTCTCAGCCATGGCAAGTGTTCACGAAAGCCTATACTTTAACCCCATGATGACTAA
CGGGGTAGTTCACGCCAATGTGTTCGGTATCAAGGACTGGGTGACACCATACAAGATCTCAGTGCTGGTGCTGCTGAGCGAGATGACCAAAGATACGAAA
ATTAGCTTGGTGGAGAAGAGGCGCTTAAACAAGCAAATCCTTCCCCTCTTGCAGGGACCAGATATGACTCTCTCAAAATTAATTAAGATTGTTGAAGAGT
GCTGTCCACATGTAGCAAGCTCTGTCCAGATCAGAATAAAGTTGATGGCTGAAGGAGAACTGAAGGACATGGAGCTATTCTTTGATGATCTTGCAGATTC
TTTCACAGGAACTGAACCTGAAGTGCATAAAACAAGTGTTGTTGGACTTTTCCTTCGACACATGATCTTGGCATACAACAAACTTTCCTTCAGTCAGGTA
TACAAACTGTACACATCATTGCAGCAGTATTTTCAGAGTGAGAAAAGCAAGGCCACTTCAAAGCCAGATAGTAATGAGGGAGACATGGATCTTAGTAACA
CCGAGGAGGAAGAAGGAAAACTAGAGAAAGAAGATCTGGATTTTCCTATTGGGGAGGAAGACTTGGCTTGCAACGGCCCTTTATCTCAGAAACAAGCAGA
ATATTTCCTGTCTCAACAGGCTTCTTTACTGAAGAGTGATGAGAGCAAGGCCCTGCCCCCAGTTTTACTGCAAAAGGAACTGAATAATTTGCTGAAATTC
AACCCTGATTTTGCAGAAGCTCATTACTTAAGCTATTTAAACAGCCTACGTGTTCAAGATGTGTTCAGCTCAACACACAGCCTCTTGCATTACTTTGATC
GTTTAATTCTCACCGGTGCAGAGAGTAAAAGTAATGGAGATGAAGGATATGGGCGGAGTTTGCGATATGCTGCACTGAATCTTGCTGCCTTGCACTGCCG
CTTTGGACACTATCACCAAGCGGAACTTGCACTTCAGGAGGCAATCAGGATAGCACAGGAGTCCAGTGACCATGTGTGTTTGCAGCACTGCTTGAGCTGG
CTGTATATCCTGAAACATAAGAAAGGTGTAGACAGCAGTGTTTTATTGGAACATTCTGTGAAGAAAGCCATACACTTTGGACTACCATATTTGGCCTCCC
TAGGCATACAGTCCTTGGTTCAGCAGAGAGCTTTGGCAGGGAAGACAGCTGACAAACTCATGGATGCCCTGAAGGACTCTGATCTTCTTCACTGGAAACA
CAGCCTGTCAGAACTTATTGACATCAGTACAGCTCAAAAGACAGCAATTTGGAGACTGTATGGACAGAGTACCATGGCACTTCAGCAAGCCCAGATGCTC
CTTAGTATGAACAGCTTGGATGCTGGAAATGTTGGGGTTCACCAGAACAACACAGAATCTTTTGCTGTGGTTCTCTGCAATCTTGCTGAGCTTCATGCAG
AACAGGGTTGTTTTTCAGCGGCTTCCGAAATCTTAAAGCATTTAAAAGAGCGATTCCCTCCTAATTCCCAGCATGCAAAGCTTTGGATGCTGTTTGACCA
GAAAATTCAGTTTGACAGAGCAATGAATGATGGCAAATATCATACAACTGAGTCACTGGTAACTGGCATTACTGCACTAAACAGCGTGGAGGGATTGTAC
AGGAAGGCTATTGTCTTAAAAGCTCAAAACCAAACTGCAGAAGCCCATAAAATTCTACAAGTGCTGCTTAGTCACTGTCAGAAGATAAAGAACATTGAAA
TGGTGATTCGAGTCCTGCTGTCCTTAGCAGAGCTATACTGGAGGTCTTCCTGCCATCCTGTAGCATTGCCAGTTTTACTTAAGGCCTTGGCTCTGTCACG
AGAATATAGACTCCAGTCTCTAGCTTCAGAAACTGTGCTCAACTTGGCTTTCTCTCAGCTCATGCTAGGAATTCCAGAACAAGCATTAAGCATACTACAT
ATGGCAATTGAGCCCATTCTGGCTCATGGTGCTGTGCTGGATAAAGGTCGTGCCATGTTTCTGGTCGCCAAATGTCAAGTAGCTTCAGCTGCATCCTACA
CTCTACAAAAGAAAACTGAAGCTTTGGAGTCAGCCATTTTAAACCTTAATGAAGCAAAAGCATACTTTGCAATGGTGGACTGCAAAGAGCAGATCCGGGA
CATCCTGTACTTACAAGCTAGGCTGTACAACACTTTGGGGAACATACAAGAAAGGAACAAATGCTCAATGCTTTTCCGCCAGATGCATCAAGAGCTTCCT
TCTCATGGGGTATCATTAGTTATTCGCCTGTAGGAATGTTGCTGCCAAGTGATACAGTACTGTGACTGCTCCTGTACACAGCAAATCCCACTTTCTGGGG
GTAAATTGCCCTGGACCATTCACTGGTGGGATATTGGTCTCGAAGGAAGACCTTCTGGATAAAGGTTATGCAATGCATAAAGATGTCCTCTTGAAAGCTA
CTTCATAAACAGTGATTTTCAGGACAAGTTTTTTTTCAGATGAATTTTTCAGATGAATGTATGCAGCTGAATGCGGCACCTTTTTCTACAGCCTACCTGA
GAGCTTTGCTGCCTTTATTTCAAAATATCCATTTCACCGGATTCTGTTATGGTAGGGCATACAGATCTCAGCCGAACAATGTAGTGGAATATGTAGTGTT
CTCAACATTATGAACTTGTTTATATTGGTATGGGAAGTACTGTCTGGGGTGTTTAAATTAATTTTTATGCTAAAAGTGTATTCACTGTTAAACCCGCTCT
CGAGCAATATCCACTTCATAAATTAAATATGCAAGTTTTCAGTAAAAAAAAAAAAAAAA
5' EST: CF783196 [+]taattaatccccccccccccccgctgttgagtgtatggcaaagtggtgcctgagacggagcttctgtctcagccatggcaagtgttcacgaaagcctata
ctttaaccccatgatgactaacggggtagttcacgccaatgtgttcggtatcaaggactgggtgacaccatacaagatctcagtgctggtgctgctgagc
gagatgaccaaagatacgaaaattagcttggtggagaagaggcgcttaaacaagcaaatccttcccctcttgcagggaccagatatgactctctcaaaat
taattaagattgttgaagagtgctgtccacatgtagcaagctctgtccagatcagaataaagttgatggctgaaggagaactgaaggacatggagctatt
ctttgatgatcttgcagattctttcacaggaactgaacctgaagtgcataaaacaagtgttgttggacttttccttcgacacatgatcttggcatacaac
aaactttccttcagtcaggtatacaaactgtacacatcattgcagcagtattttcagagtgagaaaagcaaggccacttcaaagccagatagtaatgagg
gagacatggatcttatttacaccgaggaggaagaaggaaaactagagaaagaagaatctggattttcctattggggaggaagactttggcttgcaaccgg
cccttttttctccaaaacaagcacgagtattttccttgtcttcaaaaaggcttttttttactgtataaattgttagaaaagccaagggcccttggccccc
ccgttttttcctggcacaaaggaaaactgaaataaaattttggccctggaaaattcacaacccctggaaattcgtgtccaaaaaaacctcctcttttacc
ctttaaaggac
3' EST: CF783195 [+]tttttttttttttttactgaaaacttgcatatttaatttatgaagtggatattgctcgagagcgggtttaacagtgaatacacttttagcataaaaatta
atttaaacaccccagacagtacttcccataccaatataaacaagttcataatgttgagaacactacatattccactacattgttcggctgagatctgtat
gccctaccataacagaatccggtgaaatggatattttgaaataaaggcagcaaagctctcaggtaggctgtagaaaaaggtgccgcattcagctgcatac
attcatctgaaaaattcatctgaaaaaaaacttgtcctgaaaatcactgtttatgaagtagctttcaagaggacatctttatgcattgcataacctttat
ccagaaggtcttccttcgagaccaatatcccaccagtgaatggtccagggcaatttacccccagaaagtgggatttgctgtgtacaggagcagtcacagt
actgtatcacttggcagcaacattcctacaggcgaataactaatgataccccatgagaaggaagctcttgatgcatctggcggaaaagcattgagcattt
gttcctttcttgtatgttccccaaagtgttgtacagccctagccttgtaagtacaggatgtcccgggatctgctctttgcagtccaccattgcaaagtat
gcttttgcttcattaagggttaaaaaggctgaactcccaagcttcagttttcct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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