XB-CLONE-4354095
Clone Name: IMAGE:7026528
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Gene:
fscn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783202
[+]
taattaatcccccccccccccgctgaagagagactttgggctagcggaacgggcttgagcatcttactggcaacatgacttctggaccccttcaactcgg
gctcgttaactgcaacaacaaatacctcactgccgaggcttttggattcaagatcaatgcttcggcaagcagtttaaagaaaaagcaagtgtggagcctg
gagccagcgggtgaagatactagtgccgttttacttcggagccacctaggccggttcctttctgcagacaaagatggcaaagtgtcgggggagagtgaga
ctgctggacccgagtgccggttcctggtcagtgcgcagggggacgggcgttgggcgctgcagtccgaggcgtacggccggtacttcggaggttcagagga
tcgaattagttgtttctctccttcggtttcccctgccgagaagtggggagttcacttggccatgcacccacagttcacactatatagcgtgacccgcaaa
cgatatgcccgcctgagtgccaatggcgacgagttatccgtggaacgggacgtgccttggggtgtagactcgctcattactctcattttccaggaaaatc
gctaaagcatacaaccccctgatcatcgcctgttggcctccgatggcacccttaaaaacgggcccggtcccggaaaccggttataccctggacatttcat
tcgggaaaagtggcttttaaaacccaangaagggcgttacatgaacctctttctggccccaagtggaaccctgtaaaagcagggcaaaaaaaaacaaagg
ncccgggaaaaaaaaaaaacttgttttgtcctggaaaaaaaaaatcttgcccccaaaggggggtttcctttcatgggcgggggaaaacgcgggaaggaaa
aacttgtttctcttcccaagggccggggggtatatttttattttt
3' EST: CF783201
[+]tttttttttttttttggacaaaatgtatttatttctctacactgcgatgttctgaaaatggcacttaagacattataatcagcatgtggcagctttacag
atcaattcacaggagtgcgaaaccaccgagcctttagtcacatctgctcactgaactggtgttcagatcagtggtacagcagacagattaaatagcccag
attaatatacaaacaataccacgtattcaatctttgatatcaactgttggtgatacattttatgacatttgagttattttaaaatttagagcattgtcat
ttagcaccacaaagtatattaaattcactgccgaaataatatgtcaaacattgagatgaggcttatcagtaaacataaaagtaaataaaagatatttaat
actgtttattactattaatattctacctttccaattacataaacctttctacagggaggaatatggccctatgaaatactaggcataaattgttaaatgt
gcaagtttcgcattattttaaaaaagcaaacatttttttcaaatagctagcacttggcaaattatgaccactgccacttaaagggcctcctaagacatga
cattttcttttctcctcatcaagactggtaaagactaatatagttccttaaagctagcaaggggggtcaatccccggaaatccctacccgagtttaaaaa
acctttgttaagaaacagttcctgttaatgggatggtaaaccaaattttaaaatatgacctggggtcaaaatttatccccattctggcatttccctttaa
agggatccctttttaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: