XB-CLONE-4354092
Clone Name: IMAGE:7026526
|
Gene:
dact1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC080457 [+]
CCCGGGAGTGTGTGGCGGTGAGAGTGCTGCGGTGCTGTCATGGGCTGTTTCGCTTAGCTGCTCGCCAGAGATTCAGCAAAGATGCGCCTAGCACTGCGCA
GGAGACGCTAAATCGCACGTTGTGCAAACTTGGTGCATTGTATCCGCTGACAAGTGGGAGTTTGGGATCGGTTCTTGCGGAATAGTGAGGATAAGGATTA
CCTGCTCATTGGAGCGCCCTTGTTCCTTTCCAGCCTGGCTGCCTTTGCAGATGCTCTAGCTTGTGGCTTGCTCGCTGTCCCAGGATGAAGCCGATCCCCG
CCACCCCTGACCACCTGGGGCAGCACCAGGAGAGCCCCCGCCGTAAGGACAAGGGAGAGGCGGAATCGGAGCGGCAGAGGACCCGGGAGCGCCTGGAGGC
GACCTTGGCCGGACTTGCGGAGCTGGGCTATCTCAGGCACCGGCAGGAGCTGCTGGTAAAGAGCGCACTTACCCCCGGGGCACCGACTCACGGAGATACT
GCCACTCGGGCAGGGGACACCCCCCGCAGCCTGGAGGAGAAGTTCCTGGAGGACAATATCTTGCTGCTAAAGAAGCAGTTGAACTGCCTTAGAAAGCGAG
ACGCGGGTTTACTAAGCCAGCTTCATGAGTTGGACAAGCAAATAAACGATCTCAAAATTGATGTTGAAAAGACAGAGGAGCATCTGGAAACAGACAGTCG
ACCCAGTTCAGGATTTTATGAGCTGAGTGATGGGACGTCGGGATCCCTCTCCAATTCCTCCAACTCAGTGTTCAGCGAATGTTTATCCAGCTGCCACTCC
AGCACCTGCTTTTGCAACCCCTTGGAAACATCATTAAACCTCACAGATGGTCAAGCAAAGTCTGCAGATGACTTCCTTGAATGGTTGGATTACAGAGACA
GTCAACATGAAACGGGCACAGTTCGCCGTTCCTTTTCTGCACCACATTCCAACTCTGTTGACGTTGCAGCAGATGTTCACCCAAAGTATCAGTGTGATCT
GGTATCTAAAAATGGCAACGATATCTACCGTTATCCCAGCCCACTTCATGCAGTAGCTGTGCAGAGTCCTATGTTTCTTCTTTCTGTTATGGGCAACATA
AAGGCAGAGGAACCAGAGGAGGAGATTGGCCCCAATGATAATGATGACTGTATAGTGCCTGAACTAGACCACTTAAAAGATGAAGACTCTTTTCTCCATC
AAAGCAGCCTGTGTTCTTTACCACTTTCTTCTGGGAAAAAAATGGATGGGTACATATTAAGCATCATACAGAAAAAAGCACATCCTGTAAGGACTAACAA
ACCAAGGACTAGTGTCAATGCTGACCCAGGCAAAGGAATTTTACGACATGGGAGTATATGTGTCAAACAGACTGGTGGTGTTTCTCAAAGCAGCACAGTT
AACCTGAAAAATTCTAAACAAACATGTTTACATTCCACCGGAATGATCTCTGTGGATAATAGCACTTATCCTTCTCTAAAACAGTGCTCAAAGGAGTCTC
TAAGTGAACAGCTGGAAAGTAAAAGGATGCCATCCATCTCCACGTATCCTTCTTGTAATGTCAATGAACTTCAAAGCCAAAACAATTCACGAAATACTGT
GAAGTCTGTGTGTCAGGGTCTGGCAAGGGGCTCTGTAGCAATGACCTCAAATGTTCAAAAGGAAAATGTTACGCCCAATGCACCAACTAATCTCTCTAAT
GCATCAAGTTCGGCCTGTAATGGATCTCCTAGAGAAAGTACGCAGATGAGTGCACTTCTTCCACAAGAGATTAAAGTTGTGCCACCAGTGAAAAAAATTT
CCCCCAAAAATACACTCCTTTCTTATCATGCATCATCCTCTTTTGATGAAAGACCACCTTTGGATTTTAAGAGCGAGGGATCGTCATCTCAGAGCTTGGA
TGAAGGGCTGCTGGTCAATGCTCATTATATACCAGCTCAGCAACAGGGTGTTAAACTTCATAAGAATATCAAAAATGTGAAGATTGTGAAGAGCTCCACT
TTAAAACATAGGGCTAATGTTCAGTATGTGGCAGAAAATGGGTCCCAGACGCTGAAAGAAAAATCCAAGGCTGTGGGAAAGAAGTGCAGGTTCCCTGAAG
ACTTGGATACAAACAAAAAAGTGAAGAAATCCACTCCACGGACTAAAAAAACGTCTCACCCTAATTTTGAACCTGCAGTAGTTGGAAGAAATCCAGTTGC
AGTCAGATCTGGAATCAAATCTCATGGACAGCCAAAGGATGTGGTCTTGGCCAAACCCAAACATAAGAGAGGTGACTATCGGAGGTGGAAGTCTTCAGCT
GAGATTTCTTATGAAGAAGCATTAAGGCGGGCACGAAGACGAGCCCAGAGAGAAATGATGGGTGTTTATGCCCAAGTGCCGTTTCCTTACGCCAGCCCAT
ACGCTTACATTGCCAGCGACTCTGAATACTCTGCCGAGTGTGAGTCTCTGTTTCACTCCACAGTTGTAGACACCAGTGAGGATGAACAGAGCAATTACAC
CACAAACTGCTTTGGGGACAGTGAATCCAGTTTGAGTGAGGTTGAATTTGTTGGTGAAAGTACAACGTCAAGTGATACGGATGAAAGTGGTGGACTCATA
TGGTCCCAGTTTGTACATACCCTTCCCATGCAGGCCACTGCAACGGCTGAACTACAAACCACAGCAAAAGCCTTTGTTAAAATCAAGGCATCACACAACC
TTAAGAAGAAAATCCTCCGCTTCAGATCTGGATCTTTAAAGCTGATGACAACTGTTTGAACATCACCTTTTCACCAACTCCAGCTCCATTTTATTTTGTA
TATAATTTGTTACTTTTGTGCAAATATTTAAAGTCTATGGTAAATTAAATTGACTCTCTTGAATATCCTTGTGGACTGTCAAAATTCCAATGACTGGTGT
AAATCCACGTAATTTATCTTTCTGCTTACATTGTGCGCTTAAAGTGTCCAACATCGATGTTCTCTGGCAATCTGCAGATGTCCAATTAGACACATCCAGA
ATAGACCACCCAAGACCAGTGCAAACAGCAGTTCTAGAATGCTTGGTAAACATTCCATTAGAACCAGCAGCTTTTCATTTAGAAAATGGAGTGAACTTTT
TTTTTTATTTTTAACTGCATGTACTTCTTTCCAGCTAGAGCTGAAGTGGTTTAGAACTAGTGGTCCATTCTGAGCTTTCCAAACCGTGTGTCTTTCGCAA
TTGGAGTATAATTCCCCAGTTTCTCTCTTCAAACCTATGCATGCACTGTGCATGAATCTCATTGGTTACCATATGCCTTTCACTATGGAGTTCATGTATA
CTTTAATCACTTGTATTTTATATATTTTGTAAATTATATAGCACATTTTACATGGTAATAAATTGTCTGCTTTAATGCTGTTTAAATTTATTAAATTGAA
ACAAAACGAAAAAAAAAAAAAAA
5' EST: CF783204 [+]taattaatccccccccccccccccgggagtgtgtggcggtgagagtgctgcggtgctgtcatgggctgtttcgcttagctgctcgccagagattcagcaa
agatgcgcctagcactgcgcaggagacgctaaatcgcacgttgtgcaaacttggtgcattgtatccgctgacaagtgggagtttgggatcggttcttgcg
gaatagtgaggataaggattacctgctcattggagcgcccttgttcctttccagcctggctgcctttgcagatgctctagcttgtggcttgctcgctgtc
ccaggatgaagccgatccccgccacccctgaccacctggggcagcaccaggagagcccccgccgtaaggacaagggagaggcggaatcggagcggcagag
gacccgggagcgcctggaggcgaccttggccggacttgcggagctgggctatctcaggcaccggcaggagctgctggtaaagagcgcacttacccccggg
gcaccgactcacggagatactgccactcgggcaggggacaccccccgcagcctggaggagaagttcctggaggacaatatcttgctgctaaagaagcagt
tgaactgccttagaaagcgagacgcgggtttactaagccagcttcatggagtgggacaagcaaataaacgatctcaaaattgatgttgaaaagaccgagg
agcttctgggaacagacagtccgacccagtttaggattttaatgagctgaaatgatggggaacgtcgggaatcccttctccaaatttcctcccacaattc
atgggtttaaccgaaatggtttaatatcagagctgggccaattcccagcaaccttgggtttttgtgtgcacacccccttggtggaaaacaatctcttttt
aaaacccccccgt
3' EST: CF783203 [+]ttttttttttttttcgttttgtttcaatttaataaatttaaacagcattaaagcagacaatttattaccatgtaaaatgtgctatataatttacaaaata
tataaaatacaagtgattaaagtatacatgaactccatagtgaaaggcatatggtaaccaatgagattcatgcacagtgcatgcataggtttgaagagag
aaactggggaattatactccaattgcgaaagacacacggtttggaaagctcagaatggaccactagttctaaaccacttcagctctagctggaaagaagt
acatgcagttaaaaataaaaaaaaaagttcactccattttctaaatgaaaagctgctggttctaatggaatgtttaccaagcattctagaactgctgttt
gcactggtcttgggtggtctattctggatgtgtctaattggacatctgcagattgccagagaacatcgatgttggacactttaagcgcacaatgtaagca
gaaagataaattacgtggatttacaccagtcattggaattttgacagtccacaaggatattcaagagagtcaatttaatttaccatagacttttaatatt
tgcacaaaagtaacaaattttattccaaaataaatgggaacttggagtttggtgaaaaggtgatggttccaaacagtttgtcatccacctttaaaagatt
ccagatctgaaacgggaaaatttttcttcctaaagggtgtggtgaaagccccttgatttttaaccaaagggctttttgcctgggggtttggaaatttcaa
cccgtttgcaaggggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: