XB-CLONE-4354062
Clone Name: IMAGE:7026514
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Gene:
mettl2b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783223 [+]
taattaatcccccccccccccattatcttcccgttccagtacccaaactggtacggaaagccaagaaggacaagtcatgcagctgaatggttgtcaggaa
gaaacagagcgagcagatgtagaaaatcctttcccaggggcctctgctacataccgcattatggaggtgggatgtggcgtgggcaacacggtctttccaa
tattgcagaataatacagacccaggcctctttgtgtattgctgtgatttctctagtacagcggtggaactagtaaagagtaatgagctttacagccctac
ccgctgctttgcctttgtccatgacgtctcagatgagcagtcttctttccccatgcccgagcacagcttggatgtcattgtcctgatattcgtcctctct
gccatcaaccctgctaagatgcagaatgtcatctctcgcctcagttctctgctgaaacccggagggtgcatccttcttcgggactatggccgctacgaca
tggcgcagttacgcttcaagaaagggcgctgtctggctgagaatttttatgtgcgaggagatgggacccgaatttatttctttacacaagatgaccttga
cacccttgttcatcagtgctgggctacagaaagtgcaaaaaacagtggaccgcccggcttcaggtgaaataggggcaagcagcctgaacatgtaccgggt
tgtgggatcccaaggcaaaaacctttaagcccgcaaggagaacaaaactgccagaaatgggtgggctgttttctttccgaaataaaaatggctgtgccca
attgtgggccaaaataaaaatggaacaaggccccccagttttcaaacttggggtgatacatatgcaaccccaaaagagggaacacaaaacccccgaacct
tgggttaaaaaaatattggaaaataaaacccagaggggggaac
3' EST: CF783222 [+]tttttttttttttttaaatatcacaaagtctttattaaaaactttattcttttaaaagcaagcatttgtccaaagtgaaaaagtgggtctgcgaaaaagt
gcaagctcaaacatgagcacataaacgtgtacccaagaggcctggcaatgccctgcatttccttggtgggacccagagatagattaaaagacaaggaata
atttaaaagactttgcgcatactgtctgtctgtataggatgtcagcctgcttaacacaaagctgcacaatccctcgctctccatatttacagtctgtttt
gtcctctcgtacatgatcacagttgaactgggctgtcactttatttgccacatgtgcagccattttattcggaagaacagccaccattctgcagttctgt
ctcttgcggcttaacgtatttgcattggatccacacccggtacatgggcaactgcttgcccctattcacctgaagccggcgggccactgtgttctgcacc
ttctgtagcccagcactgatgaacagggtgtcaaggtcatcttgtgtaaagaaataaactcgggtcccatctcctcgcaaataaaaaatctcagcccaac
agcgcccctttcttgaagcgtaactgcccccatgtcgtaacgggccatagtcccccaagaaaagaggcacccctccggggtttccagcccaaaaaactga
ggcgaaaaaaataaaatttttgccttccttaacaggggttgaatggcccgaaaaagaacaaattattcagggaacaatggaacttctcaaccttggtgct
ccggccatagg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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