XB-CLONE-4354037
Clone Name: IMAGE:7026503
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Gene:
ndufab1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077661
[+]
GGCCGTGGTGCAGCTTGGGTAGCAGCTGTCCGCCATGGCTTCCCGTGCCCTCTGTGTGTGTGTCCGCGCTGCTCTCCGTCCCCTCCCCCGGCGAGCTTTG
CCGGCACTGAGCCTTGCTGCATGTCGGACACGCTCCTCCTTGGCACGGCCATCTACGGCCGCTCCTATACTCGTACCGCAGGTTTCCACCTCCTTATTTC
AGTTATGCCGCCAGTATGGAGACCTCCCTCCCCTCACGCTGGAGAACATCGGCGAAAGGGTTTTATATGTTTTGAAGTTGTATGACAAGATCGACCCTGA
AAAGCTCTCAGTCGGCTCTCATTTTATGAAAGACCTTGGCTTGGATAGTTTGGACCAAGTAGAGATAATCATGGCAATGGAAGACGAGTTTGGCTTTGAA
ATTCCAGATGCAGACGGGGAGAAGCTGATGACACCGCAGGAGATCGTAGATTACATTGCAGATAAAAAAGATGTCTATGAGTGAAGTACAAATGTCAGTC
TCCTTGTTCACTGATGCGCTCTTTCTGTTGGTAGCTCGGGTTTGGCGCATCACAACCGCGCCTGCATGTCGTGGCACAGCTGCCCCCCAGCACTTCTTGC
AGGATCCTGTATTTAAAACTGTCTGAATATGGTGGTCTTTAATAAATGTAAAAAGGAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF723038
[+]taattaatcccccccccccccggccgtggtgcagcttgggtagcagctgtccgccatggcttcccgtgccctctgtgtgtgtgtccgcgctgctctccgt
cccctcccccggcgagctttgccggcactgagccttgctgcatgtcggacacgctcctccttggcacggccatctacggccgctcctatactcgtaccgc
aggtttccacctccttatttcagttatgccgccagtatggagacctccctcccctcacgctggagaacatcggcgaaagggttttatatgttttgaagtt
gtatgacaagatcgaccctgaaaagctctcagtcggctctcattttatgaaagaccttggcttggatagtttggaccaagtagagataatcatggcaatg
gaagacgagtttggctttgaaattccagatgcagacggggagacactgatgacaccgcaggagatcgtagattacattgcagataaaaaagatgtctatg
agtgaagtacaaatgtcagtctccttgttcactgatgcgctctttctgttggtagctcgggtttggcgcatcacaaccgcgcctgcatgtcgtggcacag
cttgcccccaacactttctgccggatcctgtatttaaaacctgtctgaaatagtgggtctttaataaatgtaaaaaggaaaaaaaggagtaatataaaag
agagggtcccccttttttctaaaagcggcccgccaccgccgtgggaaccttggcccgttggcccccatttggtttttatggcccccttttaaatgggttt
tccaaataaatgaccataagcctcttccccaatattttctacaaaatttaagcatcatttttttttttccttgtacatttttataaattgattgggtttt
tgggcccaaaaacatccatatctcaagggggatactcttaaaatcaaggggc
3' EST: CF783239
[+]tttttttttttttttctttttttcctttttacatttattaaagaccaccatattcagacagttttaaatacaggatcctgcaagaagtgctgggggcagc
tgtgccacgacatgcaggcgcggttgtgatgcgccaaacccgagctaccaacagaaagagcgcatcagtgaacaaggagactgacatttgtacttcactc
atagacatcttttttatctgcaatgtaatctacgatctcctgcggtgtcatcagcttctccccgtctgcatctggaatttcaaagccaaactcgtcttcc
attgccatgattatctctacttggtccaaactatccaagccaaggtctttcataaaatgagagccgactgagagcttttcagggtcgatcttgtcataca
acttcaaaacatataaaaccctttcgccgatgttctccaccgtgaggggagggaggtcctccatactggcggcataactgaaataaggatgtggaaacct
gcggtactagtataggagcggccgtatatggcccttccaaggaggaacgtgtcccacatgcatccaggctcattgtcggcaacaccccccggggggaagg
gaccgaaaacaccctcgtacacccatcccaaatggcactgggaaagccatggtgggaaagcttgctaccccaatctgcaaccctggtccggggggggggg
gggttatctaacctacctccgagtggcatcaaagacccaagttacctttggcactcccggcccccggaattaacccccacggcgccctttagggcacctt
agtaccctca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: