XB-CLONE-4354026
Clone Name: IMAGE:7026498
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Gene:
cirbp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783246
[+]
taattaatcccccccccccaacccccccgacttcgcattcggcttccaacttgtgttgtatcgtccgaagatcatagaacgtctcaccatgtcttgtgat
gaaggaaaactctttgtcggtggtctgaattttgaaaccacagaggaaagcttggagcaagtgtttagcaaatatggacaggttgctgaagtggttgtgg
tgaaggatctggaaagcaagagatcctgaggctttggctttgtcacatttgaaaatcctgaagatgccaaggatgctatgatggcaatgaatgggaagtc
tgtatatggccgtcaaatccgtgttgatcatgctggccagtcccccaatgataggaaaggtggttaattcatgtggcttcttctgaaagcctaatggttt
ttctcgggaagcatattttcaaggtgtgtggtgtgatacaaatgtattatgttgtgtaatcggccctcttttgataaatcaaaaattagaaaggcttaaa
aaaaagtctaatgaaatctctatgggcatgtatctcatcgtggccgtgtagctccatgaagaaagccttaatgggtggaatatgtattacctggaaagga
attccntttatttttagtaataatcttatgagattcattccgtcttatctttaacaccctgtataactgcagtatcacatcagatttattctttgtggag
cttctaattagtgataagtatttgttgtttacaaagagtagggttattaagaatacctttcaactataaaccaaatagattgtttcgtcggtagganacc
aatatacaagcccctatcaaactttatgtcgtttatagtgttgcctctttttgtaacaccaatatctccaaaatacttgtaggtggatctctcccacccc
ccatctatctctcctttcctcatttcttatttacgacccaccccttcacagtagatctactctcatatatctaatcaacattatatcacccctcttaaac
aatataattatcctgggaatagataagcccttctcaggtgtttttcgtttcttggggccactcttangattttgcaggaaatacaattaaatatatatta
ttacaagtaatatggattataattaaccactatcctctcggtctttctctatatctacagctgtatctcgttcatatgtcactcaccctatatatgtcta
tagtatatttccttacgcgtatctatatctataaatctgtaatggtgctatacttttcataacactctcaagatatattacagtaagctatatatcttgt
gttgttatgttctatgcgtttgacaggtg
3' EST: CF783245
[+]tttttttttttttttcttttcacattggtttttttaataggcttcgtgaagccaatcaaaagtagaatacaattccttagccacttcagtacaatagttg
catgtttagtaaaaaaaggacaatataaacaaacattttaagaacttcttctgatattgttactggagatgcgaacactattcactttgtgtatcaagga
ggtgtagccactatatacagcgcacccaagctctgccttgccttgttacactaaaggaatgcaatttaagtggactcataaaattaacttttcatgtaac
caaaaaatacagcagcttaaagttctttaaacaatgctacaggaagacaggcctagcagtctgctactaatacaggtgcttctctgcccatcaaccaaca
gcacccatccgcttaggaagttacttcctagcactacttaaccagaagggggagcagccccacctgatcagcttgaatggaacagcatagtaggatagga
atatccttgcagaacacagtaactttaagaatgagattaggtgtccaaagataaagcctttgaaacacagctgtcccacttattagagaagctgtcaggc
taactaggtctattaaaaaaaaaagtgaacacaagtagatatactacacaaacgattcggcgaagctccaaaaatctttataaatacagccattggaagg
acgatcttgagtcaagaatggattttactcttgtgtagtatagctgtcataactattccctgtaggatccctccggaacgatcaccatagcctccat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: