XB-CLONE-4354024
Clone Name: IMAGE:7026497
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Gene:
btbd17
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080456 [+]
GAGCGCACTTTTTGCCTGGGAACTAACAAGGGGAAGGTGGCAAACATGGACAGGAGAACTTGGCCTTGGCACAGCGGGGTAATGAGGAGGTTCTGTGTGG
TACCTCTGCTTCTAGTTCTGGTGGAGGCAGCTCAGAAATCCGATCTAGGAGGTGATGCCTCTGCTGCCCTCATCAACCATTCCCCGATGCTCATTCAACG
CCTGCAAGACCTTTTTCATAAAGGCAACTCCACTGATACCATCCTGAGGATCCGTACCGCTAACTCAGATGAGGTGAAAGTCATTCACGTTCACCAGCTT
CTCTTAACACTTCAGAGTGATATCTTTGATGGGCTTCTTCTAAACCAAAGTGAGGTGACTCTACAGGAACCAGCTGAATGTGCTGCCCTGTTTGAAAAAT
TCATCAGGTATTTCTATTGTGGAGAGATTTCAGTCAATTTGAATCAGGCCATCCCACTGCACCGCTTGGCCAACAAGTATCACATGACAGCTCTACAAAG
AGGGGTAACTGAATACATGAAAACTCATTTTTCCAGTGAGTCTGCTCAAGGCCATGTTGTGAGCTGGTACCATTATGCCCTGAGGATGGGTGACATAAAT
CTACAAGAAAGTTGTCTAAAGTTTCTGGCATGGAATCTTTCTACTATTATGAGCAGCAATGAGTGGGTGACAGTGAGTGACAAGTTGATGGTATCCCTTC
TTCAGCGCTCTGATCTGGTTCTGCAGAGTGAGCTAGAGCTGTTTAGTGCTGTAGAAGAATGGATTAGCAAGAATAAACCAGATGCACCAGTGATTGAAAA
GGTTCTTCGGTCTATTAGGTATCCAATGATCTCGCCAAGCCAGCTCTTCCAGATTCAGAAAGAATCAGCTGTTCTGGCCTCCTACCACAATTCAGTTCAA
GACCTCATGTTCCAGGCTTTCCAGTTTCACTCTGCCTCCCCATTGCACTTTGCAAAGTATTTTGATGTCAACTGCAGCATGTTTGTTCCTCGTAACTATC
TCTCCCCTTCTTGGGGTTCTCAGTGGATAATTAACAACCCAGCTAGAGATGACAGAAGCCTAACCTTTCAAACTCAACTAGGTCCAAGCAACCATGACAC
CAGCAAGAAAATGACATGGAATGCCCTATTTTCTCCTCGCTGGCTTCCAGTCAGCCTCCGACCCGTCTACTCGGAGTCGATACCAAGCAGCTCTCAATCA
AATCGCTTAGAGGAAGGGAAACCCCGTCTTGTTGTGACGTCAGCAATGAGTGGAATGGATTTTGCAGGAGTTACATTCCAGAAGACTCTGCTGGTTGGAG
TTAAGAGACAACAGAGCAAGGTGTTTGTAAAACATGTCTACAATGTTCATCAGAGCACCGATGAAGTCTTTGACTTCCTGCTCCAGGCTGACCTGCAAAA
AAGAACCTCGGAATATCTCATTGACAACTCCCTGCACCTCCACATTATCATAAAGCCTATTTACCATTCTTTGATTAAAGCCAAGTGAAATGTAAATAAG
ACCCAGGCCTCTGGCAGAAACCCAATGTGTCTGGAATAACCACTTCCTCGCTAATATTGCCACCCAAAAAATACATATGTGATCCCCAAGAGCAAATCTG
CTGCAGGATATCTGATGTATTAAGCAGTACAGACTAATATCCCTGCCTAGCAGCAAAGTGTTTTTCTCAATGCAAGGAAGCCAATAAATGCTAACCTTTA
CCTTGGCAATACTAAGGAAATGATGTACAGACACTGCCTTTTCTCTCTTCTGTGGTTAATGCTATACAGAGGAATTTGTTTAAAAATATTAGGTGGAAGT
ATCTAAATGATCACTATTGCAATTAATTTATATAAAAAGACAGAAGACAAGAACTTCAGTACAAAGAATACAAATTCAGACTTAGGCACAGACCTGTACC
CAGGTAAATAAGACATTTTTCTTGTACCCCACTCTTGGGAGCTGTAAATGTGCTTTCCTTCATGAATTTTTTTTGCAGAGGTCTAATGTAGTCTGGCATG
GTGATTCCTAAACTGCCTGTAATAAAGAAAATTGTATAGCTAAAAAAAAAAAAAAAA
5' EST: CF723041 [+]taattaatcccccccccccccgagcgcactttttgcctgggaactaacaaggggaaggtggcaaacatggacaggagaacttggccttggcacagcgggg
taatgaggaggttctgtgtggtacctctgcttctagttctggtggaggcagctcagaaatccgatctaggaggtgatgcctctgctgccctcatcaacca
ttccccgatgctcattcaacgcctgcaagacctttttcataaaggcaactccactgataccatcctgaggatccgtaccgctaactcagatgaggtgaaa
gtcattcacgttcaccagcttctcttaacacttcagagtgatatctttgatgggcttcttctaaaccaaagtgaggtgactctacaggaaccagctgaat
gtgctgccctgtttgaaaaattcatcaggtatttctattgtggagagatttcagtcaatttgaatcaggccatcccactgcaccgcttggccaacaagta
tcacatgacagctctacaaagaggggtaactgaatacatgaaaactcatttttccagtgagtctgctcaaagccatgttgtgaactggtaccattatgcc
ccgaggatgggtgacataattctacaagaaagttgtctaaattttctggcatggaatctttctaccattatgaaccgcaatgaatggggtgacagttgga
gttgacaaatttgattgtattccctttctttcaccgctcctgaaattgggttccgccaaaaatgaacttaaaccttttttatttgccgttaaaaaaaaat
gggatttatcccgtaatttaaaccccgaattgcgccccctttggatttgaaaaaaagggttctttctcggtctcatattatagggatc
3' EST: CF783247 [+]tttttttttttttttagctatacaattttctttattacaggcagtttaggaatcaccatgccagactacattagacctctgcaaaaaaaattcatgaagg
aaagcacatttacagctcccaagagtggggtacaagaaaaatgtcttatttacctgggtacaggtctgtgcctaagtctgaatttgtattctttgtactg
aagttcttgtcttctgtctttttatataaattaattgcaatagtgatcatttagatacttccacctaatatttttaaacaaattcctctgtatagcatta
accacagaagagagaaaaggcagtgtctgtacatcatttccttagtattgccaaggtaaaggttagcatttattggcttccttgcattgagaaaaacact
ttgctgctaggcagggatattagtctgtactgcttaatacatcagatatcctgcagcagatttgctcttggggatcacatatgtattttttgggtggcaa
tattagcgaggaagtggttattccagacacattgggtttctgccagaggcctgggtcttatttacatttcacttggctttaatcaaagaatggtaaatag
gctttatgataatgtggaggtgcagggagttgtcaatgagatattccgaggttcttttttgcaggtcagcctggagcaagaagtcaaagacttcatcggt
gctctgatgaacattgtagacatgttttacaaacaccttgctctgttgtcccttaactcccaaccgccgagtccttctggaatgtaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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