XB-CLONE-4354020
Clone Name: IMAGE:7026495
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Gene:
tmed9
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084535
[+]
CCCCCTGATGGCGGCCGGTGGTGTATTGCTTCTCCTCGCTGCTTGCTTTGGCCCGGCCTTTTCTCTCTACTTCCATATAGGGGAAACCGAGAAGAAATGC
TTCATAGAAGAGATTCCCGATGAGACTATGGTCGTAGGTAATTATCGCACCCAGATGTTTGATAAGCAGAGAGAGGACTATCTGCCAGCAACACCTGGAC
TTGGCATGTTTGTAGAGGTGAAGGATCCAGATGATAAGGTCATTCTGTCTCGCCAGTATGGCTCTGAAGGACGTTTCACTTTTACTTCCCATACACCCGG
AGAACATCAGATTTGTCTGCATTCTAACTCCACTAAGTTTGCACTCTTTGCGGGAGGTATGCTGCGAGTTCACCTTGATATCCAGGTTGGGGAGCATGCC
AATGATTACGTAGACATCGCTGCCAAGGACAAGCTAAATACATTACAATTACGTGTCAGGCAACTGATAGAACAAGTTGAACAGATTCAGAAGGAACAGA
ATTACCAGAGATGGCGAGAAGAGAGGTTCCGACAGACAAGTGAAAGCACCAGTCAACGGGTCCTTTGGTGGTCCATTGCTCAGACTTTAATCCTAGTGAC
TATCGGTGTTTGGCAAATGAAACATCTAAAGGGCTTCTTTGAAGCAAAGAAACTTGTATAAACTTGCCTAGGGCTTATTCTAACAGAAGGGCTTGGCCTT
TCTGTAATCCTGTATCTTCCATCACTAGCAGGATTTCTCAGGATGGAATTGTGCGGCTGAGTGACACCCATTTGTCGAAACAGAACAATGTGTTACAGTC
ACCAAGTCTGGATTTTATGTTCTGTCTACCATAATGTTTGCCATTTTAAAATGAGATTGTACCCTGTCACCCCTGCACTGCTAGTATTAGAAGCCTGCTG
TTCCCTTTTTTACATCCTACTACAAACCTACTAAAGCAATTTTGAGAAGTAAAAAAAAAAAAAAA
5' EST: CF723042
[+]taattaatccccccccccaccccccctgatggcggccggtggtgtattgcttctcctcgctgcttgctttggcccggccttttctctctacttccatata
ggggaaaccgagaagaaatgcttcatagaagagattcccgatgagactatggtcgtaggtaattatcgcacccagatgtttgataagcagagagaggact
atctgtttttaacacctggacttggcatgtttgtagaggtgaaggatccagatgataaggtcattctgtctcgccagtatggctctgaaggacgtttcac
ttttacttcccatacacccggagaacatcagatttgtctgcattctaactccactaagtttgcactctttgcgggaggtatgctgcgagttcaccttgat
atccaggttggggagcatgccaatgattacgtagacatcgctgccaaggacaagctaaatacattacaattacgtgtcaggcaactgatagaacaagttg
aacagattcaaaaggaacagaattaccagagatggcggagaaaagaggttccgacagaacagtgaaaagcaccagtccaacggggtcctttgggtgggcc
attggctccaaacttttaatcctaagggaactatcgggggtttgggcaattgaaacctttctaaagggcctttttttgaagcaaaagaaactttgtataa
aactttgcccaaggggcttttttcctaaaaaaaagggccttgggcccctttccggaaatccccgggaaccctcccctccacctagccgggaattttcttc
aggaatggaaaatggtggccggcttgaaatttaaccccccatttttgtttccaaaaaacaagaaaaaatatggggtgtttaacaacccc
3' EST: CF783249
[+]ttttttttttttttacttctcaaaattgctttagtaggtttgtagtaggatgtaaaaaagggaacagcaggcttctaatactagcagtgcaggggtgaca
gggtacaatctcattttaaaatggcaaacattatggtagacagaacataaaatccagacttggtgactgtaacacattgttctgtttcgacaaatgggtg
tcactcagccgcacaattccatcctgagaaatcctgctagtgatggaagatacaggattacagaaaggccaagcccttctgttagaataagccctaggca
agtttatacaagtttctttgcttcaaagaagccctttagatgtttcatttgccaaacaccgatagtcactaggattaaagtctgagcaatggaccaccaa
aggacccgttgactggtgctttcacttgtctgtcggaacctctcttctcgccatctctggtaattctgttccttctgaatctgttcaacttgttctatca
gttgcctgacacgtaattgtaatgtatttagcttgtccttggcagcgatgtctacgtaatcattggcatgctccccaacctggatatcaaggtgaactcg
cagcatacctcccgcaaagagtgcaaacttagtggagttagaatgcagacaaatctgatgttctccggggtgtatggggaagtaaaagtgaaacgtccct
tcagagccatactggcgaagacagaaatgaccttatcatctgggatcctttcaccctctacaaacatgcccaagtcccaggtggttgctgggcagaataa
gtcctctc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: