XB-CLONE-4354017
Clone Name: IMAGE:7026494
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Gene:
arpc3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783251
[+]
taattaatccccccccccccgattcctgtcaaatattcaggggtgttaaagatgccggcctaccattcaatgcttatggagtcagacaccaagctgattg
gtaacatggcaatgctacccataagaagccagtttaaaggaccagcccctagagaaacaaaagacacagatatcattgatgaagctatttattactttaa
ggctaatgttttcttcaaaaactatgaaataaagaacgaggctgatcggacattaatatacataactctgtatatttccgaatgcttaaaaaaactacag
aagtgcaattctaaaggacagggagagaaggaaatgtatacactggnggattacaaacttttcctatccctggagaaaccaggatttccccctaaaatgc
aatgttatgtggaaaaccttccaaccaaaccagggaggaatgaaggtaaatgaaggggccttacccttgccagcacacctttagggccagggaaaaaaaa
gggccttgaagagcgcttttgtttgaaaaaaagggtgggtttttggaatcccccccaagaaaaccgagaaaaaaaaccctttataccaaagggggtgggg
ggggaaaaaaatgtctcttttttgtctcaaaaaaaagaaaaaaagagttttttttaataaacaaaaaaaaaatacctcccttttttccctggggcgcccc
cctcgggggggcccttttttttaacaattatggggggggggaaaaaataaaaaaaaatccccctatataaaaaagtgaagcgcttcgtctataaaataaa
atattttgtgagcgccacccattttttttttgtctcccccaccccccaactttttgttaaaatataaaacaaaaattttttttgtggccccccccctatt
ttttgttttttaccacaaaaaat
3' EST: CF783250
[+]aaaaatgataatattacaagacagtataaaatttatattgaaaaaaggacaaatgtattacaatgtgcggcaaaacgtgcagaattttgaagctcgtttt
aggtttctttcctcattttactgcccagggccagacagcgatttgttcataaactgtcttttgacaaagcatatccaccacttgctaggtttgtcggtct
gaggatcaaacaccttgtcacaaagcctcaagcctgtctcctgcctaagctgctgcaggtaggccctcattacctcatcctcctgtttgttggaaggttt
cacatacattgcatttagggggaatcctggttctccagggataggaaagtttgtaatccccagtgtatacatttccttctctccctgtcctttagaattg
cacttctgtagtttttttaagcattcggaaatatacagagttatgtatattaatgtccgatcagcctcgttctttatttcatagtttttgaagaaaacat
tagccttaaagtaataaatagcttcatcaatgatatctgtgtcttttgtttctctaggggctggtcctttaaactggcttcttatgggtagcattgccat
gttaccaatcagcttggtgtctgactccataagcattgaatggtaggccggcatctttaacacccctgaatatttgacaggatcggggggggggggggat
taatttaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: