XB-CLONE-4354004
Clone Name: IMAGE:7026488
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783259
[+]
taattaatctcccccccccccccgatcccagggaccctgagaagaacaagatgaaagccattatcctgctcgctctgctagccgccgtcctgattcagga
tataagtgcagctccggtgcagcctgagggaacagagcctaagagtcctcttcaccgtagaggttgcgttatctggtcctgctttggaaaaagagataca
gacaaacctgaggcaaatggggaaggtgaagctctggtgaaacctgaggaaactgatataagtgcagctccggtgcagcctgagggaacagatataagtg
aagctctggagcagcctgaggaaactggggaaggtgaagctctggtgaaacctgaggaaactgatataagtgcagctgcggtgcagcctgagggaacaga
tataagtgaagctctggagcagcctgaggaaactgagcctaagagtcctcttcaccgtagaggttgcgttatctggtcctgctttggaaaaagaagaaca
gaccaaccctgaggcaatggggaaggggaagctctggtgaaccctgatgaaaatgtaataaattgcaacctccggtggcagccctggagggaaacagaac
cgccaaaatcccggtcgctccccctttccgattccctttgggaaatttaaaaaccccttatgcccaacccgggggaattaaaaaacctttcaaaccaaat
tggttgaatggggattttccaaaaagggtgaaacgctaaaggaaataaagccttgagacccaggggaattcctaaaagtgttctttttttttccccattt
aaatttttccctccaaaatccccaaaaataaattaagttatcattctcccctttttcttcttcatagtggggataccgttggtcctcttttattccccaa
gggtaattgtgccctcgcccccttattttcctttattaagagggccccccccgagaaaacaccccattaaaaaaccaattccagtcgttaca
3' EST: CF783258
[+]tttttttttttttttacactctaatgatgtctttttattgatatggtaacaatagcatcaaattgtatgtatttttcattgtgtttatcaagacaaaaaa
cccaaatcccttggaaggggtcaggcagataaaaagaaagataaagaggtatcaggggccataaaggaataaggcgcggcatgccggggataaggacacg
actcacattaggaaagggagatgaactaatatttgggagttgaggaaatttaatggaaaataaacatttagagtccatggctcaggcttttccttagctt
cacatctgaaatcacatcaacatttgttgaaggttctaatccccggttggcatagggtcttaattagcaagagatcgaacgggacgacggattttgcgct
ctgttccctcaggctgcaccggagctgcacttatatcagtttcatcaggtttcaccagagcttcaccttccccatttgcctcaggttggtctgtatctct
ttttccaaagcaggaccagataacgcaacctctacggtgaagaggactcttaggctcagtttcctcaggctgctccagagcttcacttatatctgttccc
tcaggctgcaccgcagctgcacttatatcagtttcctcaagtttcaccagagcttcaccttccccagttttcctcaggctgctccagagcttcacttata
tctgttccctcaagctgcacccngagctgcacttatatcagtttcctcaagtttccaccaagctttcacttccccattttgcctcaagtttgtcccggat
ccctttttccaaagcagggacca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: