XB-CLONE-4354001
Clone Name: IMAGE:7026486
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Gene:
myl1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783261 [+]
taattaatcccccccccccccaccacacgctccaagccctcggtcggacatccctcagctccctgctcgtctccccaagccaagctcaacatgtctttct
ccgctgatgaagttgctgacttcaaagaggctttcctgctctttgacaggacaggagacagcaaaattgccctcaaccaggtagctgatgtgatgagagc
tctgggacagaaccccacaaatgctgaggtcaagaaggtcctgggcaacccctctgctgaagagatgaacgccaagaagattgagtttgagcagtttctg
cccatgatgcaggccgttgccaacaacaaggaccagggctcctttgaagactttgttgagggtctccgtgtctttgacaaggaaggcaacggcactgtta
tgggtgctgagcttcgtcacgtactggccactctgggtgagaagatgagggaggaggaagtagaagctcttctggcaggtcaggaagactccaacggctg
tatcaactatgaagcctttgtgaaacacatcatgtccgtctaagcggatatctcaggaactgtcacaagaatgtctcagaacaccgggagcaccagaact
gtacaaaaaccagcaactggttacaactaactatacggaactgaactgtacaatacaccccccatttttttttttatttttcatcactggaatttcaaca
tttacttttcatggaaccctggccaatattttaaaacgggatcaaacaggaacaatggcaaccccccgacaaaaattgggggggcggaaatcctttctag
gcaaaattctggggtacccattacccctggggagcccactggtaaaaaaaaaaaaaaagaaattgtttaaattaaaaacacaccttttggacctaaaatt
ttggggggggggcccaaccaaaaggactcctttttttttattttttaaaaaaccggcgggcct
3' EST: CF783260 [+]tttttttttttttttcttttttttttttttttttttttcttttaataaaactttatttttttgaatacagaaataaaataatttaaccactccactatca
gaagggccagttactggaacctctggtagtgcaaaattgcagtgatcttgcaaaactggcccaaagcctttggcaggttaaataaaaagacttggtagcc
accaaattagcaaaggtgtttattgacatgtctttttttttttacagtgctccatggtatggtacacaaatctgcctaaaaaatttcgccaccaactttg
tcgggggttgcatcgtcctgtttgatccgttttaaatattggcagggttcatgaaagtaaatgttgaaattccagtgatgaaaaataaaaaaaaaatggt
gggtgtattgtacagttcagttccgtatagttagttgtaaccagttgctggtttttgtacagttctggtgctcccggtgttctgagacattcttgtgaca
gttcctgagatatccgcttagacggacatgatgtgtttcacaaaggcttcatagttgatacagccgttggagtcttcctgacctgccagaaaagcttcta
cttcctcctccctcatcttctcacccaaagtggccagtacgtgacgaaactccgcacccataacagtgccgttgccttccttgtcaaaaacacggaaacc
cctcacaaagtcttccaagaacccccgggtccttgtttgttggcaaagggcctgcatcatggggcaaaaaactggctcaaactcaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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