XB-CLONE-4353999
Clone Name: IMAGE:7026485
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Gene:
ndufs8
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077660 [+]
GACAATCTCGCGTGAAGAGACTTATGGTGTCTCGATTTTGTGTATAAGACTAGCAAGGAGCTTGCAGTTAACATCTTTGCAAGGGGGACTGTGAAGATGT
TGGTTGTACGTCTGATCCAAAATGCTGTTAAACGGCCAGGTGTTTCTTGTACTCAGATGTTGTCAAGGCCTCTCAGTCTCGCTTCACAGATGCATTCATA
TAAATATGTTAATGCTCGAGAGGAGGCAACAGATCTGAAATCTGTGACGGATCGTGCAGCCCAGACCTTACTTTGGACAGAGCTTTTTAGAGGCCTAGGA
ATGACCCTGAGTTATCTGTTCCGGGAGCCAGCAACTATTAATTACCCATTTGAAAAAGGTCCTTTAAGTCCACGGTTCCGTGGTGAGCATGCATTACGTA
GGTATCCATCCGGTGAGGAGCGCTGCATTGCCTGCAAACTCTGTGAGGCAGTCTGCCCTGCACAGGCTATTACAATTGAAGCAGAGCCACGTACTGATGG
AAGCCGTCGGACTACAAGATACGACATTGACATGACAAAATGTATCTATTGTGGGTTCTGCCAGGAGGCATGTCCTGTCGATGCAATTGTGGAGGGCCCA
AACTTTGAGTTCAGCACAGAGACTCATGAAGAACTTTTATACAACAAGGAGAAACTTCTGAATAATGGAGACAAGTGGGAAGTTGAGATTGGAGCAAATA
TACAAGCTGACTTTCTTTACCGTTGAAATCTGTCCCTTCAGCTACTTCAGAACACTCAAGCCCATGAATAGCGGATAACTTGTGTGAGCCGTTATCATCT
CTAACATGGTCATCCTTTAGTATGATTACACTTCAGAGTACTTAGTTATATTTTGGAAAATTATTGTAAAGAAATAGAGGAACATTTGTGCAATTGTCTT
TGTGAATATTAAATTTCTTGTGTTCCAAAAAAAAAAAAAAAA
5' EST: CF723046 [+]taattaatcccccccccccccgacaatctcgcgtgaagagacttatggtgtctcgattttgtgtataagactagcaaggagcttgcagttaacatctttg
caagggggactgtgaagatgttggttgtacgtctgatccaaaatgctgttaaacggccaggtgtttcttgtactcagatgttgtcaaggcctctcagtct
cgcttcacagatgcattcatataaatatgttaatgctcgagaggaggcaacagatctgaaatctgtgacggatcgtgcagcccagaccttactttggaca
gagctttttagaggcctaggaatgaccctgagttatctgttccgggagccagcaactattaattacccatttgaaaaaggtcctttaagtccacggttcc
gtggtgagcatgcattacgtaggtatccatccggtgaggagcgctgcattgcctgcaaactctgtgaggcagtctgccctgcacaggctattacaattga
agcagagccacgtactgatggaagccgtcggactacaagatacgacattgacatgacaaaatgtatctattgtgggttctgccaggaagcatgtcctgtc
gatgcaattgtggagggcccaaactttgagttcagcacagagactcatgaagaacttttataccaccagggaaaaacttctgaataatggagaacaattg
ggaaatttgaaaattgggagccaaataatacaaagctgaacttttctttttcccgattgaaaattctggtccctttcagcctaactttcaggaaaacact
caaagccccaatggaaataggccgggataaaacttggtggtggaagcccggttttatctaatcttccttaaaat
3' EST: CF783262 [+]tttttttttttttttggaacacaagaaatttaatattcacaaagacaattgcacaaatgttcctctatttctttacaataattttccaaaatataactaa
gtactctgaagtgtaatcatactaaaggatgaccatgttagagatgataacggctcacacaagttatccgctattcatgggcttgagtgttctgaagtag
ctgaagggacagatttcaacggtaaagaaagtcagcttgtatatttgctccaatctcaacttcccacttgtctccattattcagaagtttctccttgttg
tataaaagttcttcatgagtctctgtgctgaactcaaagtttgggccctccacaattgcatcgacaggacatgcctcctggcagaacccacaatagatac
attttgtcatgtcaatgtcgtatcttgtagtccgacggcttccatcagtacgtggctctgcttcaattgtaatagcctgtgcagggcagactgcctcaca
gagtttgcaggcaatgcagcgctcctcaccggatggatacctacgtaatgcatgctcaccacggaaccgtggacttaaaggacctttttcaaatggggaa
ttaacagttttgcggctcccggaacagataatttcgggtctttcctaggcctttccaaagcttctgtccaaagtattggtctggcgtttccacgatccgt
tcacaaaattttaagatctggttgctccccccctgatgccttaacattattttcaaacacaaggccttccgttgaaaccctattacgcgacccgcccttt
tcactcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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