XB-CLONE-4353981
Clone Name: IMAGE:7026478
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Gene:
eno1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783273
[+]
taattaatcccccccccccccgctccccagtcgttgccggaagagaacggttgtacctacaagtagaaagtaaaaatgtctatcaagaagatccttgcac
gtgaaatttttgactctcgtggtaacccaacagtggaggttgacctgtacacatgcaaaggtctcttccgtgctgctgttccaagcggagcctccactgg
catttatgaagcactagaactccgggacaatgataagactcgctacatgggaaagggtgtggctaatgcagttgagcacatcaataaaaccattgcacct
gcactgataggcaagaatctaagtgttgtggagcaggaaaagatcgacaacctcatgattgaaatggatgggactgagaataaatccaaatttggtgcta
atgcactcttgggagtgtccctagcagtgtgtaaagctggagctgcagagaaaggagtcccactgtaccgtcatatcgctgacctggctgggaacccaga
ggtcatccttcctgtaccggcatttaatgtgataaacgggggttctcatgctggaaacaagctggctatgcaagaattcatgatcttgcctgtgggtgca
gacaccttttaggaggccatgcgcataggagcagaggtgtatcataacctaaagaatgtcatccaggctaaatatggacaagatgccactaatgttggtg
atgaaaggagctttgctcccaatatctttggagaataaagaaagccctgggagcttctggaaaacctgccatcaacaagggctggctacccagaataaaa
gttgtaaattgggaatgggatggttgcacccttctgaaatttcctacccgggaacgggaaaaaaatgaaactggggactttcaaggttccccctggaagt
acc
3' EST: CF783272
[+]tttttttttttttcccttcaaagagtttattgcctcttcgtgtctagagtgtgacaaaccattatctagtctgaaactgcaataggatgagaacacaaga
ctacaaagttgacatgaccaggaccactgccacaatagaaaagacaaggactacatgcaggcaccagtgtgacactgaatttggaataatcctaagcagc
aaagcttttaaactggaaagacagcaaggttatgctgggagaggctgtaacatctaacattgctggaagtgcctgaaattaaatgagggctcagcagtga
catgtaagacctcctgtagtctctcctaggtgcacagctactacatcagttgggtgagagcctaaggatgcctccatttagttgaaaacaggttttctga
aatttcttccagcaaagcgggccttgctgccaagctcttcttcaatcctgagcaattggttgtattttgcaagtcgctcagatctgcatggagcacctgt
cttgatctgtccagtgcggagaccaaccaccaggtcagcaatgaaagtatcttctgtctccccagagcgatgactgaccatcacaccccagccatttgac
tgaacccacttgcacgcctgcagggattctgtcactgtgccgatctggttgaccttcaaaaccaaacggttacaaaacttctcatccaccgcccttggga
aatacgtttagggggttgggtccctggtcaagtccttccccaacttacctggaaaattcccaaaaaaggcaggtaaacatttttttccaagggctttcca
agttat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: