XB-CLONE-4353972
Clone Name: IMAGE:7026474
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Gene:
ldha
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082741 [+]
GACTGACTCACTGACTCGGAGGGAGAAGCCGCGCAGGAGCAACATGGCGACCGTAAAGGATAAACTCATTCACAATCTGCTCAAGGAGGAGTCGCTCCCC
CAAAACAAGGTCACCATTGTGGGTGTCGGGGCCGTTGGCATGGCCTGTGCCATCAGCGTCCTGCAGAAGGATTTGGCAGATGAGCTTGCACTCGTTGATG
TGATTGAGGACAAACTAAAGGGGGAAATGATGGATCTGCAGCACGGCAGCCTCTTCCTCCGTACCCCCAAGATTGTGTCTGGGAAAGATTACAGCGTCAC
AGCAAACTCCAGGCTGGTGGTGGTGACGGCGGGGGCCCGGCAGCAGGAGGGGGAGAGTCGCCTCAATCTGGTTCAGCGCAACGTCAACATCTTCAAGTTC
ATCATTCCCAACATCGTGAAGTACAGCCCCCACTGTACCCTCCTCATCGTCTCCAACCCCGTTGACATTCTGACCTACGTGGCCTGGAAGATCAGTGGTT
TCCCCAAGAATCGCGTGATTGGCAGCGGCTGCAACTTGGACTCCGCCCGGTTCCGTTACCTCATGGGGCAGAAGTTCGGGATCCACACCCAGAGCTGCCA
CGGTTGGGTCATTGGGGAACACGGAGACTCGAGCGTGCCAGTCTGGAGTGGGGTGAACGTGGCGGGCGTCTCCCTGAAATCCCTGCACCCCGACATGGGC
ACGGAGTCGGACAAGGAGAACTGGAAGGAGGTGCACAAGCAGGTAGTGGACAGTGCCTATGAAGTCATCAAGCTGAAGGGCTACACCTCGTGGGCGATTG
GCCTGTCCGTGGCCGACCTGTCCGAGAGCATCCTGAAGAACCTGCGCCGGGTCCATCCCATCTCCACCATGATCAAGGGCATGTACGGGGTGAATGACGA
CGTCTTCCTCAGCGTCCCCTGCGTCCTGGGCAACTTGGGCATCACCGACGTGGTGAACATGCCGCTGAAGGCCGATGAAGAGGAGCGGCTGCGCAAGAGC
GCCAGTACCCTGTGGGCCATTCAGAAGGAGCTGCAGTTCTGACCCGTTGGTCCAACTTGCACTAAGGATTAGGTCAATGTGTGTAGTGTTTGTGTCTCTC
AAGCCCCTCCCACCCCCGGGCAGATCCCGCCCACAGAACAAACTCCTCGGGGGAGTCTGGGTAATGTCCCCACTTCCATTGGACCAGAATGGGGGGTACC
GGGGGGGGAACCCATTTAGAATGCACTTTCCTGAATGTCTCAGGTTCAACCATTCGCCCAAAGAGAAATCTATATAATAATATATGCCGCCATTTTGTCT
GTGTGACTCCGCCCCCTTGTGTCCCCGCCCACTGTCTCTCGTCCTCCTTGTTCCCATGTGACAATAAATATTGGACCTGCCGCCTGGTCCCAACTGAACC
CCAAAAAAAAAAAAAAAA
5' EST: CF783278 [+]taattaatcccccccccccccgactgactcactgactcggagggagaagccgcgcaggagcaacatggcgaccgtaaaggataaactcattcacaatctg
ctcaaggaggagtcgctcccccaaaacaaggtcaccattgtgggtgtcggggccgttggcatggcctgtgccatcagcgtcctgcagaaggatttggcag
atgagcttgcactcgttgatgtgattgaggacaaactaaagggggaaatgatggatctgcagcacggcagcctcttcctccgtacccccaagattgtgtc
tgggaaagattacagcgtcacagcaaactccaggctggtggtggtgacggcgggggcccggcagcaggagggggagagtcgcctcaatctggttcagcgc
aacgtcaacatcttcaagttcatcattcccaacatcgtgaagtacagcccccactgtaccctcctcatcgtctccaaccccgttgacattctgacctacg
tggcctggaagatcagtggtttccccaagaatcgcgtgattggcagcggctgcaacttggactccgcccggttccgttacctcatggggcagaagttcgg
gatccacacccagagctgccacggttgggtcattggggaacacggagactcgagcgtgccagtctggagtggggtgaacgtggcgggcgtctccctgaaa
tccctgcaccccgacaatgggcacggagtcggaaaagggagaaactggaagggaggtgcacaagcaaggtagtgggacagtgccctatgaaagtcattca
aagctgaaaggggctacaccttcgttggggcggattgggccctggttccgtgggcccgaacccggtccccgagaaagcatttcctgggaagaaaccctgg
cggccggggggtcccac
3' EST: CF783277 [+]tttttttttttttttggggttcagttgggaccaggcggcaggtccaatatttattgtcacatgggaacaaggaggacgagagacagtgggcggggacaca
agggggcggagtcacacagacaaaatggcggcatatattattatatagatttctctttgggcgaatggttgaacctgagacattcaggaaagtgcattct
aaatgggttccccccccggtaccccccattctggtccaatggaagtggggacattacccagactcccccgaggagtttgttctgtgggcgggatctgccc
gggggtgggaggggcttgagagacacaaacactacacacattgacctaatccttagtgcaagttggaccaacgggtcagaactgcagctccttctgaatg
gcccacagggtactggcgctcttgcgcagccgctcctcttcatcggccttcagcggcatgttcaccacgtcggtgatgcccaagttgcccagggacccag
gggacgctgaagaaaacgtctttcattcacccccgtacatgcccttgaatcatgggtggaaatgggatgggacccggcgccaggttcttcaggaagcctc
tcggaaaggttcgcccacggaaaggcccaatcgcccacgaagggggacccccttcaccttggagaattttcataggcacctgtcccccacctgttttgtg
gcaactcccttcccattttctcccttgttcgaaattccggaccccaatattccggggttcaggggtattttttggggaaacctccctccccccgtgttcc
cctcccaccattcagaatagggaaacgctctccgaatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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