XB-CLONE-4353964
Clone Name: IMAGE:7026471
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Gene:
zc3h10
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081358 [+]
GGTGGCGTTGGATCGATTGTAAGTGAGGAGGCCGGGAGCTTGCGACTAGCGTATTTGCGGGAGCCCTTTTACGCAGAGTGCTACTCTTTGCCTGGTACAG
TGCACACGCTAAGGCAAAGCGCAAGCGTCGGATTAGAAAATCCCATATTAGTAAGAATCAAGCGCTGCTAAAACAATGCCTAACAGGGAAAATGTAGCAA
GTTGTAGCAGCAGCGAGGAGGCAGCAGCTGTTGTGGAACATGTTTGCCGTGACTTTCTGCGCAATGTATGCAAGCGTGGCAAGCGCTGCCGCTTCAAGCA
TCCAGATGCTGACGATGTATCTGACCTTGGTGTTCAGAAGAATGAGTTTGTGTTCTGCCATGATTTTCAAAATAAAGAATGTGTACGGCTGAACTGCCGC
TTTATTCATGGTACAAAGGATGACGAGGAGCACTATAAGAAAACAGGAGAACTCCCACCTCGCTTAAGGCATAAAGTAGCAGCTGGATTGGGCTTGTCAC
CTACTGACTTGCCAAACAAGGATGAGGTACCTATCTGCCGTGATTTCTTAAAGGGTGATTGCCAGCGTGGGGACCGGTGCAAATTCAGACACTTGCAGCG
GGAATATGAAAACCAGTATGATTATTCCTGTGATATGCGGGGAGGGATGATGAATATGGGGGTAAGCCCTGGAAGCTTATCTGCTCCTACTACTCGTACC
TATGAGCAATATGGCAATTATGATGGTCTTACAGACACAGAATATTATTCTTCTTACTACCGTTATGGGGACCGATTTGATGATCCTGTTATGAAAAGAA
GACGCATGGGATATGATGGATATTATAGTGCTTCTCCAGTAGAGTACCGTCTCCTAGAAGAAGAGAATGTTATGCTCCGTAGACGTGTTGAGGACCTTAA
AAAGCAAGTTTCTGTTTTAGCAGCAACCAATGAGGTTTTACTAGATCAAAATGCTCAGTTTCGTAGCCAGGCCAAAGTTGTTACACTTAGCACACTGAGT
ACTACAGCCCCAACCAGTGAGCAAGGTGTGGGATCAGTCACCAACTACAACCATGGCATTGCCCAAACCCATACAACTCTAAGCAGTCAGGCTTTGCAAC
CACGAGCTGTTACTCAGCAGGAGCTTGTGGCTTCTTCTGGTGCACCTGCCTCTGCTCAAAGCAATGCACCCCAACAGCTAAACCCAGAGATAGCCCCACT
ATCTGCAGCGCTAGCGCAAACCATTGCTCAAGGCATGGCTCCTCCTCCAGTATCCATGGCTCCAGTAGCTGTTTCTGTGGCACCAGTGGCTGTTTCAATG
GCTCAGCCCTTGCCTGGTATCACAATGAGCCATGCTACTACTCCTATGGTTACCTATCCTATAGCCTCACAGAGCATGCGCATAACAGCCTTGCCTCACT
GATTAGCAAAACTGTAATAGCCACAGTGGCTTATATCTTATGTGGCGTGGTCCTGCCAGTAATAGGCATAACACTTTTTTGCCTTTCATTCTGTAACATG
GGAACACATAAGTAATACACTTCTGGTATCATGTTGTATACCATCATTTCCACAACATCATAAATATTTTAACTAGGCGTTTTTAAAGGCAAACTATCAT
AATACCAAATTGAAAATTATAAATCCCTTATGTAGTATAATTTTCTTAAATGTTGTGCTTTTTTCTAATTTTGTAGAGAATAGGTTTTTGTCCCTCTACA
TTCCATCCTTACTACTGGCAGCTCTTCTGCTTTTCTGCATGTTTGCACATCTGCAGTGTGGGGTTATACTGAAGTGCTGAGCCCTGCAACTTGTTAATAA
GCATTACATCATCTGCACAAAATTATGACAATTACAAGACCAAGATGTGAGAGGAGCTAGGATTTTCTATAAAAACATTTCCCCTATTGACTCAGTAGTG
TGCCTGTTCAATCGTCAAGAACTTAGAAAGCTGCCAGTAGCAAGGCGGAAAGTTTAGCAGAGCAGTGCAAACAGTAAAACTAATGTATTATCTTCCTATT
TATAAAAAATGCTTCTTTCCGATCAAGCTATTTATGCTTTGGTTTTGTGATAGTTCCCCTTTAATATTCTTAAAGATCTACAGAATAAAGCCTAAGTTAA
GCTGTGCTCGTCTATGGACATCAAACAAGTGCTGGGCTAAAATACTGCTCAATACATATCTGTTGCACATATTTCCAGATTTTTGTTTGACCTTTACTTT
AAAAAGAAACTGTACAATTGGTAAAATCAACATACATACATACAAGAGAAACGTAGTATGTATGCGCCATTTCTTTGAGCTAACTTGTTTTACATTGGGG
TCTTGCCTTTTATGTAAGGAGCCTTTTCTCCATAATAGGTTTAAATGTTTTGACGCTTCTACATCTAGTTTAATTTAATATGTATAGAAAGGCAACCATT
TTTTACACAAAATTAAATGATTGTTAGGAATTTCTCTGTAGTCCTTGTGCTGTGCCATCTGTGTAATGCATCTCAGTAGTTGTATCTATTGGTATTTCAT
TTTATCCTCCAATATTTCCAAAGGCGTTAAACAATTACATTAGGGATTTGGAGGGCTGCCATTTACAGTTCAGTGCAACGACTGCTCTCTAGGTCCTCTA
CTAACTTGTAATAGAAATATATAACCCATCATTTTGCCATTAAGGTAAAATCCGATAAATGTTTTCACTCTGCATGTCAAACACCATCTGCTTACCCCTT
CTACTCTTACCTTTGTTTTTGTTGTGTGTAATAATGGCTCAAGTTTGAAGAAGAAATTGAAAAATTTGTAACAATCTTTGATTTCTGTCATAAAAGTTTA
AACACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF783283 [+]taattaatcccccccccccccggtggcgttggatcgattgtaagtgaggaggccgggagcttgcgactagcgtatttgcgggagcccttttacgcagagt
gctactctttgcctggtacagtgcacacgctaaggcaaagcgcaagcgtcggattagaaaatcccatattagtaagaatcaagcgctgctaaaacaatgc
ctaacagggaaaatgtagcaagttgtagcagcagcgaggaggcagcagctgttgtggaacatgtttgccgtgactttctgcgcaatgtatgcaagcgtgg
caagcgctgccgcttcaagcatccagatgctgacgatgtatctgaccttggtgttcagaagaatgagtttgtgttctgccatgattttcaaaataaagaa
tgtgtacggctgaactgccgctttattcatggtacaaaggatgacgaggagcactataagaaaacaggagaactcccacctcgcttaaggcataaagtag
cagctggattgggcttgtcacctactgacttgccaaacaaggatgaggtacctatctgccgtgatttcttaaagggtgattgccagcgtggggaccggtg
caaattcagacacttgcagcgggaatatgaaaaccagtatgattattcctgtgatatgcgggggagggatgatgaaatatgggggtaagccctggaagct
tatctgctcctactactcgtacctatgagcaatatgggcattatgatggtcctacagacacagaatattattccttcttactaccgttatgggggaccca
tttgaaggattcctgttaagaaaaagaagaacgcaggggatattgaagggatatttaatagtgcttcttccaataaagtaacccgtctccctaaaaagaa
gaa
3' EST: CF783282 [+]tttttttttttttttcttttttttttttttttttttttttttttttgggggttaaacttttatgacagaaatcaaagattgttacaaatttttcaatttc
ttcttcaaacttgagccattattacccacaacaaaaacaaaggtaaaagtaaaaggggtaagcaaaaggtgtttgacatgcaaagtgaaaacatttatcg
gattttaccttaatggcaaaatgatgggttatatatttctattacaagttagtaaaggacctaaagagcagtcgttgcactgaactgtaaatggcagccc
tccaaatccctaatggaattgtttaacgcctttggaaatattggaggataaaatgaaataccaatagatacaactactgagatgcattacacagatggga
cagcacaaggactacagagaaattcctaacaatcatttaattttgtgtaaaaaatgggtgcctttctatacctattaaattaaactagatgtagaagcgt
caaaacatttaaacctattatggagaaaaggctccttacataaaaggcaagacccccatgtaaaacaagttagctcaaaaaaatggcgcatacataccac
gtttcccttggatggatgtatgttgaatttaccaattgtacagtttccttttaaagtaaaggtcaaacaaaaatctgggaaatatgtgccacagatatgg
tattgggccctaattttagccccccccccttggtttgaagtcccctaggacgaaacaccaggcctaaaccttaagggcttttatttcctgggaaaaatct
tttaaagaaaaaatataaaaaggggggaaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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