XB-CLONE-4353955
Clone Name: IMAGE:7026467
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Gene:
apoa1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783288
[+]
taattaatcccccccccccccgacaggagcaactaaccagcaaagcaggttggagagtttgcaactatgaagaacctagtcctactagcagtactgttcc
tctcaggaacacaggcaaagtatttcccttggcagcatgaagagccccacactccagcccagcaggtgcgggaagtcttagagagctacgtacacaaagt
tcgggacttgggcagagaagctgtgagccaactggagacctctgatctcggaaaacaacttgacctgaagatcactgagaagcttgacacctttggtacc
aatgctatgaacctgagaaagcaaataaatccctatgtagaaaatatcagggaacaggtgtccaaagagctagaaaaggaactcccagtaatcaaggaga
aaatccgccccatcctggaagctttccagaaacgtgggctgatgatgcaaaggcttaccaaggagaaaatctctccccctggtagctgaccttcaaaagc
aaaacaaagacaacccgggattcccttggaataaaaaaatcaccccctaattgctgaaaaaaaataagggaaaaaaattgaaaattgggatgttttaact
cccctttccgctcaaggggttcccacccccttacattgggaaaaaggttcaaggggtaaaacccttggggctccaaaaaaacccttttaaaaaaaggctt
ttaggggggcccttattgccccgggggtccccacaaaaaacttgaaaat
3' EST: CF783287
[+]tttttttttttttttgtatactaatgtctttattttcacattgaaacatgagagaggaccatgtgccctcagggcaatttatatgaaatatgtaaacaat
aaaagatatgtagcaattcatttcatatcaatattggttgccctcttggagataatgtaagggctagcagcatgtaaacatgtcctctctcaggccagca
tcatggtaatagtataagttgagataataggtggttgtgtgagcgagaagtttggatgaggtttgaagcaccaacatacatcttcacagatagaaggtga
aggttttaggcaccctgtctttggagcttggctctcagattttcccaaatttttgccaggttgtttctggcctcctctgcatggggtaccaggcgttctc
tgatgctctgggccaggggtccatacttctccttcaaattctcaatatgctgtgacagttgggctctgtactcctcagctctgggacctgcataggcctt
aagctcttctagtttctcgaccagctttaccctgacctcttcactgtagggtgtgagccttgagcgaagggagtcaacatccactctcactttgtccctt
atctcttcagcaataggagtgatctttttatacaaggaatccaggttgttcttggtttgcttttgaagctcagctaccagggggagagaattttctcctg
gtaagccttttgcatcatcagcccaacgttttctggaaaagcttcccaggaatggggcggattttcttcctttgattactgggnaagttccctttttcta
ggctcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: