XB-CLONE-4353949
Clone Name: IMAGE:7026465
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Gene:
krt12.5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783292
[+]
taattaatcccccccccccccacacattctgttttgtgcagactgagttgtcaggcattctcttccaataacagtcaccatgtctctgcgttctagctct
tcttattctgtacagagcaaaagtgtctctggaggtggtggttttggcagcggctatggggctagctttggtggaggaagctttagtggaggaagctatg
gtggcagcagctttggtggtggctttggagcaggctttggtggtggtgcagcttctgggttttccgctagttccgcaggtggttttggagcagcatccgc
tagttccagcttcagcagctttggcggaagcgacaagcaaaccatgcagaacctcaatgaccgtcttgctgcctaccttgagaaagtgagagccttggaa
gctgccaatgctgacttggaactgaagatccgcgagtggtatgagaagcaaagaacctctggcattggagcaggggctaaggatttcagcaaatactttg
atatcatcactgatttgaggagcaagatcctttttgccaccattgataactcaagagttgttctgcaaattgacaatgccaaacttgctgctgatgactt
cccgtctgaaaatttgagaaatgagctggcctcttcccccaaaacctgggaaggctgaaaataattgggcccgggcgttaaaaatccctgggaaaaaaac
ctttaaccccggggcttaaaaggggggaaccctgggaaaaaagctccataatctagaaaagccttttttgcaacttaaaaaaaaacaacctgc
3' EST: CF783291
[+]tttttttttttttttcaaagaactggcttggaaagctttattgcatcttgaaatcaacagaaatttccattttgtgagtgaagatttcagagatgaatct
ttatttgacgttctcttcgacttcctcaactcttgatgagacaacctttccatcgaccacttcttccacaatggtctttacttttctggtttttgatgga
tcttttttggagctggaagatgattgtgatgtgattacttgcactgatgaactggaagaactcaattctccctccagcaggcgcctgtatgtttcaatct
ccatctccagcctggtcttaatgtcaaggagctgcttgtactccatgttctgacgttccatgtcggacctgatctgcagaagttgttcctctaggcttct
gatactgagctggatggtttgaagctgggcaccatagcggccatctgtttctgccagagagtcttcaagagattttttcattgccagttgagattgcaat
tcaatttccaagctctgaaggctgcgtctgaggtcggatatttcgctcttgcttgactgcactagttcccacgcctgtttaaaatctctttttcttcagc
ttcaacacttctttcgggggttgaaaaccccagggctcttgggaatcccccctttgggggttttctcccccccccccaccccccggggggtttttttaaa
aaagccgggggccccctcccgggggggttttttccccaaaaaaaggggggtggtgggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: