XB-CLONE-4353933
Clone Name: IMAGE:7026459
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Gene:
acly
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783302 [+]
taattaatcccccccccccccccaaaatgnaagntttagaaggtgggagacggggagcaggtcatttaagagcagtcgtccatatcacattactgagagc
aacaatgtctaagggaccagcagttggaattgatcttggcaccacttactcatgtgtgggtgtcttccagcatggcaaagtggaaatcattgccaatgac
caaggaaacgggaccacacctagttatgtggcttttacagatactgagagattgattggagatgcagctaaaaatcaagttgccatgaaccccacaaaca
cagtttttgatgccaagagactcattggccgcagatttgaagatgcagttgttcaggctgacatgaagcactggcctttcaatgttatcagtgacagagg
aagaacctaggtccagggtaaatataaaagcatagaccaaatctttctatccagaagaaatatcatctatgggctgaggtaagaagaaggaaaaagcgga
cgcatacccttggctagactggaacagatgctggaaatgtactggtcccagctgtattttaatgaattcctcagccaaggggcctatgagaggaaggccg
gggtaactataatcgggggcgtgggatggtaactggcggaaaaccattgggagggggagacaaaactggcgggcttggccagtttggccattattgggaa
cttttcatcaacaaaggggctgggagatcacaacatagataacccattgctgtcatctcctttttacgccttgggcgggggggggcccccttttttgggg
gttgcgcctgatggtcagagccagatgggagaggagggggtatcttttttgggaggggtaagtttcagacccgcctgggggattctcactcattgtttgg
gaggggagatagaaccactttggaaaaacccctaaggggcgacatgactgttttgttgtggcagcagatgttgagagggagaagccagcacagaaagggt
ggtctgaattgagggataggagaga
3' EST: CF783301 [+]ttttttttttttttggatgctgttcccatttaataaatacagctctcaacacaagttaatgtcacagttttctctacacatatcaaaataaaataaaatt
aaacacagaaccaggactgggagcaggacataaggaaaaacaaaaaatttatatatatcagaatgttaagacaatgaaacagcggctatgactgtactta
gtttgcactgattggatgcaggggctatagtgctaaaagcctgcgccgaaggggtgttaatcatgtgatcacagcacaaacgcgccttctctccggagtg
tctttcaagtctttacatagtcatgtgctcaggcagaacgtaggaaatatcgtcccatggatgccggtacaatccctgcttcagccttttctggtccaag
taatgcccgataaaacccatgcttcttcccaaaacaaagatgccgttcagtgctccaatatctacgtattcatccgcttcctcccgtgtgaaagtgccgt
atgtcctgagcaaatctacaaacgccaccccgataaagccatcaacgttaaggatgaggtttggtttctttgaaattgtgatcctttccaccccaagggc
ataatcccgtaaaggagtggccgggaaacttctgcttgaccgaagtcctatcagggacttgaacccctcaatgtctgggcttgtttgaatacaatatgaa
ttccggtgccccaaatgtcacattgaaacccccctttcccccttctctttccttcaaaaccttgggttcaccaaaactacacaatgggagaaaaataaac
gagaaccgccccttgaacccaaatcaccccctttttatttttggcaaacaacaacctattcgggggacccaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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