XB-CLONE-4353925
Clone Name: IMAGE:7026453
|
Gene:
srfbp1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC084513 [+]
CCCCCCCGACATGTGATAGGCAGCAATGGAGCCAGTGCTGAATCTGAATAATGAGGTTGTAAAGTTGAGGAAAGATGTGAAGAAGGTTAAAGTTCTGATA
ATTCGCAAACTTACTCGACACATCGCAAAGCTGAAATCTAAAAAGGGGACAGAAGAACTAATCCTTAAGAATCAGAGGAGAGCCCAGAGATTGCTTGAGG
AAATCCATTCTGTTAAGGAGCTCAAACCAGATGATGTTACTAAAACTGCTTTAAGGAAAGAAATCAGTTTTGAAAAAGTATGCAAAAAGCCTAACAGCAC
TGCTGAGGAGCGAGCATTAGCAAGACTGGCCACACACCCTTTGCTTAAGCAGAAAATCACAGCTATTAAAGAGGCTATTAAAGCTTTTAAAGATGCAAGA
AAAACTGCTGCTGAAGGGGAGAGGGAAAGAGAGAAGGATGAACCTGAGCAGGTCACAAAGATTAAAGAAACCAAAAAGCCTGTACAAGCAAAGCTAAATA
AAAATACAGAAGAGATAAAATCAGCAAAAGAACATGTTAAAGAAGAGAAATGCAAAAACCTGCTAGAAGATTCAGATAAGGGCACTGAAAAAGCTCTTGA
ACTACCTTATGTGCAAGAGAACTTGCCTGAGCAGACAGCAGAAAACAAGGAACAGCCGAAGGCCCAAGATGTGGAAAGGCCAGCTGTGGAAAGGCCAGCT
GTGGAAAGGCCAGCTGTGGAAAGGCCAGCTGTGGAAAGGCCAGCTGTGGAAAGGCCAGCAGTGGAAAGGCCAGCAGTGGAAAGGCCAGCAGTGGAAAGGC
CAGCAGTGGAAAGGCCAGCAGTGGAAAGGCCAGCAGTGGAAAGGCCAGCAGTGGAAAGGCCAGCAGTGGAAAGGCCAGCCGTGGAAAGGCCAGCCGTGGA
AAGGCCAGCAGTGGAAAGGCCAGCAGTGGAAAGGCCAGCAGTGGAAAGGCCAGCAGTGGAAAGGCCAGCAGTGGAAAGGCCAGCAGTGGAAAGGCCAGTT
GTGGAAAGCCCAGCAGTGGAAAGGCCACCTGTGGAAAGCCCTCCTAAAAAGAAAGCTTGCTTGGAGCAAGAACTAGGTTGTGAGTTAAGTGATATTGAAG
ATTCAGACAAAGAGAAAGAGTATTTTGATGACAGCACAGAGGAGAGGTTTTATAAGCACTCATCTAGTTTTGAGGATAGTGATAGTGGCAGCGACAATGA
CTTTTTTATTGGCAAAATTAGAAGAACAAAGAAAAAGAAGTCAGACAAAGATGGCAGCAAGCAGAAGGAAGAAAAAGTGCCACCTACTAAAGAAAAAGCA
CAAACATCAGAAGTACAGAAAGAGATTCCAACTGCTAAATCTATGAAATTAAAATCAGTTTTTTGCAAGTCTTTATCACAAACCAAGCCAAAGCCTTCCT
TTACAAAAAGAGAAACAAACTTCCGACAGGAGAGAAACAAAAGACCAGTTATGCCGCAGGCAAGTCCACTGGCAAAGAAACCATTACAGAGCAAAGCCAC
ATCTGTGAGGCAACCGGGCAGAAAACTAGAAGCGCAGCCGCTACACCCCTCGTGGGAAGCCAGCAGGAAACGCAAGGAACAGCAGGCTCAGATTACAAAA
TTCCAAGGGAAAAAGATAGTATTTGATGATTAGAATACTTTTTAAAGACTTAATATTTCTTTTACACAAACAACTCTTTTTTTGCCTGCAGCAAATTCCT
ATATTAAACAGTTAATGCATAACAAAAAAAAAAAAAAAAAAAA
5' EST: CF723048 [+]taattaatcccccccccccaacccccccgacatgtgataggcagcaatggagccagtgctgaatctgaataatgaggttgtaaagttgaggaaagatgtg
aagaaggttaaagttctgataattcgcaaacttactcgacacatcgcaaagctgaaatctaaaaaggggacagaagaactaatccttaagaatcagagga
gagcccatagattgcttgaggaaatccattctgtttaggagctcaaaccagatgatgttactaaaactgctttaaggaaaaaaaatcatttttgaaaaaa
taatgcaaaaaagccctaacagcacttgcttgaaggagcgaagcatttaccatagactggggccaccaccccccttttggctttaggccagaaacaatcc
acaggcctatttaaaagaaaggcgttattaaaagccttttttataaagaatggccaaagaaaaaaaattcggcctgctctttaaaaggggggaaaaaagg
ggaaaaaaaaaaaaaaaaagggatttgaaacccctttggagccccagggtcctccctaaataaagatattttataaaggaacaacctcccaaaatataag
ccccctggtttatttttagacccaataaagcctttttacaatattaattaaaacaactcaccccaagtaacggcagagaggattccgatatagattctct
cccccaccttagaatatagatataacattgtgttttttaataaaagctactattaacagataatatttgtggcgctaagatatcaccctccgccccccct
gataaaaaagatttttctctcaagaataatatatgggcgctcctccttcaaaaatataaaagagcccccttcttggggaaaacattactctccttttata
ttgggtgccaaaagcaaagaaaaaactctttggtccctctttaatgcccgaatataacacacttaccnatataataatataaaaggacagaaatacattc
nctatataaagagcgtctctccactaaagttaattgtgtattaaaaagagcggtcctcccttctcgttgtggtggtaataaagtggcccctcattctttg
tgggtn
3' EST: CF783306 [+]tttttttttttttttttttgttatgcattaactgtttaatataggaatttgctgcaggcaaaaaaagagttgtttgtgtaaaagaaatattaagtcttta
aaaagtattctaatcatcaaatactatctttttcccttggaattttgtaatctgagcctgctgttccttgcgtttcctgctggcttcccacgaggggtgt
agcggctgcgcttctagttttctgcccggttgcctcacagatgtggctttgctctgtaatggtttctttgccagtggacttgcctgcggcataactggtc
ttttgtttctctcctgtcggaagtttgtttctctttttgtaaaggaaggctttggcttggtttgtgataaagacttgcaaaaaactgattttaatttcat
agatttagcagttggaatctctttctgtacttctgatgtttgtgctttttctttagtaggtggcactttttcttccttctgcttgctgccatctttgtct
gacttccttttcttggttcttccaattttgccaataaaaaagtcattgtcgctgccactatccactatcctcaaaactaaatgagtggcttataaaacct
ctcccctggggctgtcatcaaaaaaacccttttccctttggtcggaaatctttcaaaattcgcttaacttcaaaaacctaatttctttgctcccagacaa
agctttttttttttaggagggggtttttcccaaagggggggccttttctcaccgggcggggggttttttcaaaaaaggggccctttttccacctgggggg
gggctttttcccatgggtcggggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: