XB-CLONE-4353922
Clone Name: IMAGE:7026452
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Gene:
tspan31
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783308 [+]
taattaatcccccccccccccgaagaatgcgctgtgtgcgctgaatgtggtgtacatgctggtgggcctgctgttgattggcgtggcagcatggggtaaa
ggttttggaatcgtctctagtatacacatcattggtggagtgattgccattggagttttccttctactcattgccataattggtttgattggggccgtta
gtcatcaccaagtgatgctctttatttatatggttgttttgattctggtctttatatttcagtttattgtctcgtgttcctgtttagcaatgaacagatc
acagcaggagtatttcctgaatacaacatggactcggatgagcaatgacacaaggctgtaatttaaaaaaaaaactctgggaatgcatgtgggttttttt
aaacacttacaaatggccatgggaaaaaagttttaaaaatgggattgttcgcttccttcgggcagttaaaaggcgacaaaggttttgggttcaccaaaaa
tcccccctcgttaaaaattgcccttaaagacttgggtggcgggggaaaaaaaaaaatagtttttaaaaaaccatattgtctatatattttaaaaataact
acccttgtgaagaaaatttctcccctctcccagataagggtttcgtttttgcgaggggcaaattactttttatttcaacttactaagtgatagcttgtat
tgtatggggaagagaagagaattctatattcccctcttatagagccatacccgcaatatagtatgaactgctttttcttttttggggcacgtctttttta
tttttctccgcgcgtgtctattacaacctcggggtggttgtttttttatttttttcataaatttagtggttggtgttttctntctccatgttttattttt
gaaattttataattatcacccctcccctcgggagggtgaagtggtattgattataatattcactttcaaattgaaagagggtatattatactcagttttt
ctacccaaacaattttttttaaatgatctattatttagcgccgtttggtggactcccccgttctctttttccgccctacctatttgtgtttggncgttgc
tgattcnccctctatttgttcacgcacacaacttgagaacttccntgcagtgcccccccctgnctctcttttcctttctctacttagacgctccccttta
gttttcagattatttttataacaacactgcgaatcgtcttattgttataagagaataa
3' EST: CF783307 [+]ttttttttttttttcacacaatatccatttaatttatgaacacttgcaaatgtgatctgattgcattcttgacggaagcttctatttcttgaggcttagc
caggggtttcacttctccacttcacatgtccctgcatgacagaacatgttgcccatagagggagaagtgatcggcaaaggaatgaggctgatacaggaga
atagactacatgattgaagagtcaggaagcgaaatagaatgaacactatagaaatgcactggggtttgcacgggggtccttctgattcctgtagcggaaa
gcaagccacacaccaagaatctcagtaaaactgaacaaaagcccaacacctccgaggattttcagtgcttcatctgcatggtttaacattttatccccac
agcttaggcatttctgagggttttgtgaacagacttgcgccttactgcagagagcgacatccattttaaactcttccctggcatctgtagtgtttaaaaa
cccacagcattccagcgttttctctaaattcagccttgtgtcattgctcatccgagtccatgttgtattcaggaaatactcctgctgtgatctgttcatt
gctaaacaggaacacgagacaataaactgaaatataaagacccgaatccaaaccaccctttaaataaagagcatcactttggggatgactaacggcccca
atcaaaccaattatggcaatgaagtagaagggaaaactccaaggggcaatcactcccccaaatgaaggggtatactaaaagacaaattccaaaaactttt
acccccgggctgcccccgccaaatcaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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