XB-CLONE-4353920
Clone Name: IMAGE:7026451
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723049 [+]
taattaatcccccccccccccagatttctctagaccatctaggagcctgaagctctctcctctgctccgaggtccgacttcagaatgagggtctgggtct
tcttcgtcttgtgcctggttggcaaagctctagctgcccctcaacaggatgctttaccagaagaggaagaggtgatagaagatgttcccacagaagagac
agtgggtgctaatcccgtccagtttaaatccgggaatttgccaacccttccttggggaggaggaggaaaagccttttgaaaaatccctgcctggacccac
ccctgcaaaaattggcaaaattttgggaaattcaattaaaaccacccccccccatgtttgggttggcaaaaaacccttttttctgggccccataattttt
accccaaattttaaaaaggttttttgggcccaaaaaaaaaaaaaccttttaaactttttcccccccccttttttttcaaacaaaaaatcccccccccgaa
ggggggcccaaaaaagggggcaaaaaaaagcgcgtttttttgtttttttttttggacccggggagaaaaaaatttttcccccccccgttttgggaaaaaa
acctccagcggaaattttttcccccccccgcataatgcgggaattagggtgcccccaaaaaaatttctcgcgggaaaacacttttttttttttaaaaagc
aagttataacccccccttttttttttttgttaaaaaataaaaccaaacaaacgtggggggggagaaaaaaaaaatcctctccctagaaataaaaaaaaaa
cctctcgtttagttatcggggagaacaccccccatctttttataataattcgggggggcgggggtnattttttatttataataaaaaaaaaacacacctc
ccggtgtggtttttcttttctttctccccccgccggcgggagt
3' EST: CF783309 [+]tttttttttttttttcatttttcattgtttattctctgagttgaatggcgtgcagtgatgtcaataaaagtaagccgaatcgtctaaataaaatatagag
acattctggcagaaatgctttataagaggggtcaggctaaaccaccaccccccttaaaaactaagcccatacccaccctttccttgttacaaaaccccca
acctcctaccccctgcatccagtcaggggttaaccccttaattgctagagggctgctccagcagatgaaggggttaaagtcctgctttaacactgtgttt
tcaggaatatataacaataggttaagacttaggatgaagatggccttatgagttggacagtctatgaaaggaaaagagaagcataactggccagacactg
gaattcagagctagtggtgacgacgctttgatctcctccgcttgctattgagttttattacttgttctatttagcaccgttaataatgttttctaagtta
gcacctttgctgcccaacctcattagaccaaattattccaaacttaaggggtgaaaacagggaaaattacaacttataatacacccctaaataaaagatg
cctcaaaccggagttcttcgggtgtttgtctttaaaacaattcattattcccttatccactttccctgttcctttgaattccaaaagcaccttggcccca
attctttcctaagggcaaaggtatatttatccgttggtgcaagtgtcactaccccatttaaaaaaaaaacgttgaagggtgccaggtggcccccattggg
gggaatctaaaaggtggccccccgaaacggggaaaaccacacccctgtggtcgggtacaagggaacccccttcgaattggggggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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