XB-CLONE-4353915
Clone Name: IMAGE:7026449
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723050 [+]
taattaatcccccccccccccgaagtattgcgcgcaaagattcaacggacaagaggcaaaaaggcaaaacgtatgggtggcttctgtttgtatttccatc
atgtagcagtgatgcagctgagagaggagtgcatggtgctgttacaagtgaaaaaataaaaccccctggacagcaaaaaaaatatctgttagggctttga
agtttccttctgaagtaaaaacacaatgggtggcggggaaaaagaaaaatgcaatctttggggcccaaaataatttttaccggttccccaaaaaagggaa
aagggggaaaaaacaacaatggcggggggcatagggtttgcaaataccccccgggggaacgctggggaggaaaatattaaaaggggggggagaaaaaaaa
aggggaaaacaccaagggaaggggggcgccgggggtgcctggccccaaaagggaaaaaacaaggggggagtgaggggcgccctttgttttccaaaccaat
aagatcgccgccaagaggtcaattattatatctcatttgtactgcatcaaggttattggtacatgagaagggagaggtataaaatgattggtgtttataa
atacatccccgaacataagatgaaaagaagagggggggggtgggggggataagaagataaatggagaaaaaagacatgaaaagttgatctagctttataa
gataaaagngatgaagaaagagaccacactattctattgtnttgtgtagtaggtagcagcgagtgatgggggagaattaaattattctgtatatnttcat
ttctctcacgtgcntattcttattatatagataaaanaagccatctcgtgatgcaggcggcgcggtgggtgcgggcgtgattatcgtgntgactttcccc
tcgagcatatgagaaaacaacgaaagaacacaacacacgtagtaaatntgaataaagcgtatanataatttgatcttgtgcacctgcgcggtactgcgaa
tttatatatatctgtcaacaccctncttacataaagatattagctaacagaactagtactaaaaagcattgcctcacatgacgcgggntgctgatacgaa
agattcctatcactaaacaccatacacacatcacgcaatataaaaaaatagataacatatatcctacatcaactaatgacagtatatcatagctaatagt
gngaacgattgtacttattaattatacccctaatataatatagggtgtaatntatatatgcactcatgcaggtgtttgtatggccc
3' EST: CF783312 [+]gttttgtttcgggaaccaatatgtctagtaagatctctctggaatgaagcaggaagtccaagcttgcttaactcaggtaatcgtggacctgatgaattgc
tagcacctttctgtagttcaaggtcagaaatagtttcaaaagtttcagcatcattaatatcttgacattgatgattaacgtcggtgtcttggttttcttc
ctcagtgcatagctcagttttgaaatgtggcctaaagctatcaatatttggttcaacagaagacgtatcaggatctgaattggtgcacaagtccaaggat
tttgctaatttttctaaagcgtcttcagttttttcatttttggcatcataatccagaacacaatcacgtggatattcattgtctaatattttacctttat
ctgaggaattctgggtatgaccaacatttccaaatgtatccttagaaacattgatggttttctggggattatttaatacattagcggttatgctttgatt
tattanaccatccctgggagcaggacagtaatgactactttgaactgcctgactattttgcagtatttctttagctttctgcaaaacttcagcaagtgtt
ttcactggctctttttggctgcagtcttgagccggtttatggtttttttatgtccttagaactgctatgcagtttgaaagtggggtctgggtttctaaat
ttaantgggctcaacctggctcgtgtcgcttcttcaaaaagtcatagggaaaaaaaatggttttggcaacctttcaaaaggatttttttccatcctttcc
ctaaacttaaaatttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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