XB-CLONE-4353910
Clone Name: IMAGE:7026447
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Gene:
ckb
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723051 [+]
taattaatccccccccccccgacacatcccttcaggtctggggcaagtgttggtcctgaggagaggttagggtctccagtttgcggtttccaattaagca
agactaagcaatatcagtatggcgcaaccaattattaagaggttgtcccttgaggaagaataccctgatctgagcaaacacaacaaccacatggctaagg
tgcttactgtggatatgtacaagaaactgaggagcaggatgacttccagtggattcaccctggatgatgtcattcagactggggttgacaatcctggcca
tccctttattatgacagtgggatgtgtcgctggggatgaagaatcttatgaagtctttaaggagctctttgaccctatcattgaggacagacatggtggc
tacaagccaacagatcagcacaagactgacataaattcagaaaacctaaagggaggtgatgatctggacccaaactatgtactcagctctcgggtcagaa
ctggaaggagtattcgtggattcagcctcccacctcactgcagccgtggagaaaggcgtgcaattganaagatgtcaattgaagcacttgctagcttgga
tggggacctaaaaggaaaatactacgctcttaataccatgactgatcaggagcagcagcagcttatcgatgaccacttcctgtttgataagccagtttct
ccactcctcttggcctctggaatgccccgtgactggcctgatgccagaggaatctggcacaacgacaacaagacccttccttgtctggaatcaatgaggg
aagaccaatcttcgttgtgatctcaattgcaaaagggttggccat
3' EST: CF783315 [+]tttttttttttttttcttgacaggtcaacgcaagaccattgtttattttctatctttagtaacattgcagccatcaaaagaaagataattctaagaaaca
gatggaagacatttctacagtgttactaagcaggttgggtccccagggtcttggagtgtagctatttcatccaataaattagaaagcatggccaataaag
tgcagttatttctgtgctggaatgaggtcatcgatggactctcctttttccaggcgtttttccatttcaacaaggagttttacaccatcaaccaccattt
ggaccagttcaacctcagagaaacccagacgatcagcattggagatatcaaacaccccaccaacggctgcagtgtctacaccacctgttcctctcttctg
tagtctaagtctcttcaggatttcaccaaacttctcattcttgcttaagtttggcaacttaatgtgaacaccagccctcagaccagtaccgaggttggat
gggcaggtaagaacataaccaagatgctggttccacatgaatgcagatcctttgttcttgaagatagctccaatctttgtaaggccagtgcaaaaacctc
tggaacacatccttcatgttgccacccttttgcattgagatcacacgaagatggtcttcctcattgatccagacaaggaaaggtctttgttgtcgtttgt
gcccagaattcctcgggcatcaagccagtcacgggccatttccaggggcccaagaaggagtgggagaaactggccta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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