XB-CLONE-4353900
Clone Name: IMAGE:7026441
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Gene:
c3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723054
[+]
taattaatcccccccccccccgaaggcagtgccctgctgggcaggatttgccaagatgatatttgccggtgtgctgaagagaactgtttcatgcagcagc
aaattgaggggaaaatcactcctgaaatgagaatcaacatggcttgtgctcctggagtggattttgtgtacaaggctactctcacggaggtgcaggagag
cgagaattttgacaactacgttatgacaattaggaaggtcataaagcaaggtgagcttgtggcatgttcatatctcaaggtctagttattctgccgttct
atgaaccctcatttcctgcttctctgtaatcctttagggacagaccaggagcctgaggagaagacacgtaattttatcagccatatcaaatgccgaaaag
ctttagccatgcagctgaaccgagattatctgatctggggggtaactggggacctgtggaagcaaccagatgggtaagtacggtggaagcctcttgagtc
agtgagtttcacccatcgacacatgcacgcatagtcaccaaagagaggcatcatcagatcagaatgtatgtcaagaccaactgcaatacaatatggatgt
tctgtttaatacatttatactgactgcccatttgagcatactccagtggaggcaggagggggttgtggcccctttcacttacagatccccccataataaa
acgtaataatgcctggttctttgtagctattcctacatcatcggggaggacacatgggatcgagtggggggcccaatgagagggaagtgcccagccaacg
tgaagaaccctgggaatctctggtgaaggatttttgaagaaccggttttctgaacaaacctgggaagaatcgga
3' EST: CF783320
[+]tttttttttttttttctttttttttttttttttttttttttttttttaagcaaataaatgcttttattggaattgtatataaaacatggcacaacattta
caaaatgttacaaaaaagcttgggaaaatggaaagatcttggtgggaaatcaggttcctcagttgggacttggtgggaaatcaggttcctcagttgggac
ttggtgggaaatcaggttcctcaattgggacttggtgggaaatcaggttcctcagttgggacatccaacgatctccaggttgtcagaaacggtctcaaaa
tcatcacagaaatccaggttctcacgttgctggcactccctctcattgggccaccactcgatccatgtgtccttcccgatgatgtaggaatagctacaaa
gaacaggcattattacgttttatttatgggggatctgtaagtgaaaggggccacaaccccctcctgcctccactggagtatgctcaaatgggcagtcagt
ataaatgttttaaacagaacatccatattgtattgcagttgggccttgacatacattctgatctgatgagccctctctttggggactatgcccgcatgtg
tcgaagggtgaaaactccctgactcagagggttccccccggatttcccccctctgggtgctttccaaaggcccccccgttaccccccaaataaaaataat
ctccggttcaccctcctggggtcaaaactttttccgggattttaaaatgggctgaaaaaaaattaacggggctctccccttcgagggtc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: