XB-CLONE-4353897
Clone Name: IMAGE:7026440
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783322 [+]
taattaatcccccccccccccccacaaaggaaagcatgtctgttgaacgtgatacccttatatactgccttatgaatctatcttcattgcaaatatgttc
tccatgaggctgagagcttcacctccttcaaacccttgaagtgtcattgccacctgctgctcatttttgttaagtggacacagtcgaaaacgaagttgat
gaaaaagcctgcgcctttcaagttattgatgctatcaagtttattctccagactattctccagactgttatgttttatgaatcacagatgctgatcttgt
gttcagttccataaagcaagcactctggtagccacccatgtttctgtagcctgccctcaagaacctagtgatgatgctgttaaaccttttctgactgtaa
tggttataaagcacagatactgatcttgcattcatatagcaagagctgtaaaagccactcatgcttatcttcaagaacctagtggtgctataaagcctgt
tctaactctggactgttagaattctaaagcacagatgctggagtccgattttgtgtgcaagagggagaacctccccagacagcctgaaatgccttcactg
tgacttctcaggtctgcagtttcatccagaaagcactgtgggtacctcccatgctatcctagctttcccaacaatcaagaaaggaaactgaacctcttac
tgaacccggaccgttttgggttaaaaaacataaattcagaggcgatttcagcttcttctttttatcctaaatcgccctttggggaaaaaccgtttcacta
ttaccaccaagtgttttcccaaagtttaacctnatttccctccaccaaggttttccaggtgtttacctgcggtattttttgtgtaaacgcccccccctct
atatggttcttatactttgtggccaattctatgtggtaaattgtctgttaaagaggaaaat
3' EST: CF783321 [+]tttttttttttttttctgaactaacaatagctcaaactgagcaaatacagcaataatgactaaaaaagtctgcaaaattgggcagccaatttgtcgaata
tcaatcaggtttccttaatgcagtaacaaaatgctcaagataagactaataagggggcgttacaaactaaccaagtaaccctgaaacatatggaagaaat
agagtaacttgtgtatacatttgtgatatagtgaacgttcttaccatgggtgactatgataagatgtagagctgaagtggatctgtatttgtgctttata
accataacggtccagagtcagtagaggctcagtatcctcacatgattgttgggaaaggctagatagcatgggaggctaccacaatgctttctggatgaaa
ctgcagacctgagaagtcacactgaaggcatttcaggctgtcaggggaggttctccctcttgcacacaagatctgactccagcatctgtgctttacaaat
cgaacaatccagagttagaacaggctttatagcaccactaggttcttgaacataagcatgagtggcttttacaactcttgcgatatgaatgcaagatcag
tatctgtgctttataaccattacagtcacaagaggtttagcagcatcatcactaagttcttgacggcacgctaaaggaaacctgcgtggctaccacaatg
cttgctttatggaactgaacacaggatcggcctctgttgattcagaaaactaacctgtctggaacaatagtctggataacaaacttgaataccatttata
aactttaaagggggcaagccttttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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