XB-CLONE-4353887
Clone Name: IMAGE:7026436
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Gene:
mypn
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783328 [+]
taattaatcccccccccccccgatgtaggacagaggaagtgccgagggtgcatgtctgtctaaatgctggatggataacccttcttgtcccttgagcagc
gccttcactaaaataggattgtttcattttcatcttcacccttggacaatttcatanaagagacngccgtgagagctggacactgacagcaatttctagc
atgncagacgagattataaagctttctcgtctattctccgatccggaaagaaaacttaatgctggaaacagaataccccaaaaaagcagtctatcaaacc
ccttcaacccattttttatccccaaaagggccggccccccaaaaaaaaaacaacttttgccgaaccttaagacccccccgggttttttcggcttttttgg
aacccggaaaaaatttaccccaaagttgtttttttttggaaaaaaaaaaccctttttccccaaatgggggaccccccaaaaagggggccccatttttttt
tttttttttgttccccccccggaaaatttttttaaaaacccccccccccccgccgcttgtttttgaaaaccccccccttttttccacccgaaaaaagaga
aattcccccccccttctctttgttgtggggaagaaaaaaaaatattttttttttttttttttttttccggggcgggttgtttttttctaaagaggggggg
ggggaaaaacactctcccccccggaagaaaaaaaaaaaatattttttttttttattaaaacccccccaagaaaaaacccccccccccctttttttggggt
gaaaaaatatatctccttataaaaaaaagaaagaaattttttttttttattaaacccccccc
3' EST: CF783327 [+]tttttttttttttttggacataaatatttaaatagggtacttatacaacatcaaacagatcacactgcatcagtgtcagtgccacgggcatacagatcac
tgagtactgctggcgcgtctgggctacatatactgcaaacctatgttaacctgttcactgccagaaagggctgggcccctctagtggcactgaaacagta
ctacctggcagttagattattttatattggcaaaacactgaagccaaacagggatctttatccccattaaaaatttgatttgtaaacgttcaagctgtat
tttcttcacagttttattttgggttttgacgtcggttccattcacagctcttcactttctaccacttcccgtgtagacaatgagtaattcagcggtgtta
ctacaggaggatgagagctgatggcatccaaagctgaaaacatagaataccgagaacctgttgatcgggtctttttgattggctggggaatctgctggta
ccactgagcataaatatcaagtcttgcagtacaggatacaatccctgcttcgttctttgctgacagtgtgtaccatcctgcatcttcttttctggcangc
tggatgaagaggcacacgtaaccagtgttatcttgatgcatgcttatccgtggtttattggccggaaagggattcattgcctttcttccagtagaacact
ggtggggggcattccaacaactcgactctccaatcctgactgggaagacctttctgggaacaccaccatttttgaaagttttttccaaaaaaactggaac
cttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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