XB-CLONE-4353879
Clone Name: IMAGE:7026431
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Gene:
hnrnpk
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783333
[+]
taattaatcccccccccccccgcttctgtttcggccgtgttggtggaggtagctcgggggtcggcgtaggagcaaggtagcagccaccaaattataagtt
gtattaaaagaagatggagacagagcaggaagaggttgaattcactaacacagaaactaacggtaaacgcccagcagaagacatggaggaggaacaggca
tttaagagatctagaaacacagatatgatggttgagctgcgtattctgcttcagagcaagaatgctggagcagtaatcggcaaaggaggaaaaaatatta
aggcgcttaggacagactacaatgccagtgtttcagtcccagacagcagtggccccgagcgtatattaagcatcagcgcagatattgaaacaattggaga
aatcctaaagaagataattcccacactggaagagtaccaacacttcaaaggaaatgattttgattgcgagttgaggttattgattcatcaaagtttggct
ggtggaattattggtgtcaaagggagcaagattaaagaacttagagagaaaactcagactacaataaagctatttcaggagtgctgccctccttctactg
atcgtgttgtgctgattgggggaaaacctgacaggtggttgaatgcattaaaagtatacttgatctcatctccgaaatcccctggtaaggggagggctca
accctatgacccctaacttcaaggacaaaactttttgaatttgggagggattttccaaggatgttttaatgaaccccaaaaggacccccccatggggttt
atcctctatgaaaggctaagaaaggtggaatttgatattaaaggccctcccggagactcggggggtgcgcccccttgtccaaggcttaggggggagtgta
tctctattggcctttagtgcccaagggggggagggn
3' EST: CF783332
[+]tttttttttttttttctttttttttttttttttttaattaaaaatgttttattgtgagaataggagaaaaaaaaaaaaaaaagtaaaaacaacctgccac
aagtgcaagagagaactaatttcaacaagttactgtacatatccctctagcgacatcaactctgcaaagatggcgtctgaaccaaaagagcctttaccat
tagaacccttgagcatgtgcttcgcaatgtttagaagtcaaaacccataagcataatcttcagaaaactgcttcacactgttctgaagcaaaaattgtgc
attctgaatctgatcttgagtgccagtgatggttattattctgtcatctgatccttccaatggctcatcaattttgatggatgcaccagattcgtggcgg
atctgtttgattctttgaccacctttcccaattatcgacccagctaaatcttttggaatggttacttgtgttgttataacaggcccgccgatatccccaa
aagaacctcgaccatatccagctccacccattccatcataatggtctggtcttcctcttcggtctcctcctctaggtggtggaggtgngaggggaaaagg
aaaattacnnaagctctacttccaccttctacccccacgaccaggggggtgggagggaagtggcccccccgccttgggactcatggtcaatcgtaatctc
cgccttggactggtggcaaagggggcgaaccaaccgggttccctgggaagggcatttccgaattaaaaccccaccctcctagccattggcattagagaaa
aacccccctgggggggggggtcccctcttttccggggtccattcaaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: