XB-CLONE-4353876
Clone Name: IMAGE:7026430
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Gene:
krt12.5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783335
[+]
taattaatcccccccccccccggcatccttgctctctgctttgtcctagctgacttgtcggagaatatctaccaataaacagccatcatgacttcctata
ggtccagctctgcttcatactattcaagctcatccggcaaaggtggctttggaggcagcagctatggaggcagcagcttggcaggcagcggctttggagg
aggctttggcagcggtgcaggctctggcttctcatcctctgggggtaactttgccatggcggcggcagccagctccagcttaagcaacttcggtggaaat
gaaaagcacgccatgcagaacctcaatgaccgccttgcttcctacctggagaaggtcagagccctggaagcctccaatgccgacctggaagggaagatcc
gcacttggcacgagaagcaaacaggcaccggccttggagctgggtctaaagactacagcaaatattttgagatcatcagtgagctgaggggcaagatcca
cggtgcaactgttgataacgctacagtgaccctgcaaatcgacaacgccagactggctgctgacgacttcagacttaagtttgagaatgagctggctctt
cgccagagcgtggaggctgatatcgctggcctgcgtagagtcctggatgagctgaccctggctagaggtgacctggagatgcagattgagagcttgactg
aagagctggcctacctgaagaagaaccatgtagaggagatgatgcatgccaagagcagaacgccngaaaagtgaatgttgagaatgacgccgctccaggc
gtggatctgaccaacattctgaataaactgagagctgactatgaaatactggctggaaaagaaccccagggatgt
3' EST: CF783334
[+]tttttttttttttttcttacgcaggtcttctctcttgtttcaggggaaatttctgttcatttgttcctttatctttcccgactgcccttggatttatgac
actgttgaagaaacaaccttcccagctaccacctcttcgacaatggtttttactgttctggttctggttgttgttgttgatgatgattctgagctggact
ccagggaacttgcctttgacactgtggtcatctgtcccatttccccatccagcaagcgcctgtatgtttcaatctccatctccagcctggatcttaatgt
caaggagctgcttgtactccatgttctgatgttccatgtcggacctgatctgcagaagctgttcctctaggctgctgatagtgttctgaatctgtagaag
ttgcgctgaatagaagccttggaattcattgaggttgccttcaactgattgtttcattgccagctgggattgcacctcaatttcccagctctgaagtgac
cgtttcagctcagtgatttcgctccttgcgggctgaacttgtttaacccccaactgaaatcccttttcttagcttccccctctttctgggtgaaacaaaa
ctttgcatccctgggggatctttccacccagtattttcaaagccagcttctcatgttaattcaaaaaggtgggttcaaatccaccccttggaaccacagt
cccaatttaaaaactttccttttttccgggccccctggggcatttgggcaagtcaaacaacctccctcctccaagggttctttctatctagggaaggccc
gcctcctttcatttcaacttn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: