XB-CLONE-4353867
Clone Name: IMAGE:7026427
|
Gene:
actb
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF783341
[+]
taattaatccccccccccccccgagtgacccgcccgcacagaaacaggacagcgtgtgtgtcgtactctcagattacaatggacgacgatattgctgcac
tggtcgttgacaatggttctggtatgtgcaaagccggctttgcaggtgatgatgcaccccgtgctgttttcccatccattgttggtcgcccaagacatca
gggtgtcatggttggaatgggacagaaagatagctacgtaggagacgaagctcaaagcaaaagaggtattcttacactcaaatatcctattgagcatggc
attgtcactaactgggatgatatggagaagatctggcatcataccttctacaatgaactcagagtggccccagaggaacacccagtgctgctgacagaag
cacccttgaatcccaaagctaaccgggagaaaatgacgcagatcatgtttgagacctttaacactccagctatgtatgttgccatccaagctgtgttgtc
cctgtatgcatctggtcgtaccactggtattgtcatggactcaggtgatggtgtaacacacactgtgccaatatatgaaggttatgctctaccccatgcc
attctgcgtctggacttggctggacgtgacctgacagactacttcatgaaaatcctccctgagagggggtacagcttcaccaccacagctgaaaaaaaaa
attgtttcgtgaatatcagggagaattggggctattgttgctcctgggaactttaagcaggaaaatgggccccaagctggctttcttcttttatccattg
gggaaaaaaatttttataaccttgccctgaatgggaactagtttattccccccctttggtatatagaactcggttttataaaatgtccccaaaaatagca
tattcttttcacaggcccctttattttttccatttgggggaataggggaaatatactcggtggtgcgcactatccctggggaagaacacc
3' EST: CF783340
[+]tttttttttttttttggtgagaaacggatgtgaaacaacttggttttatttttttcagttaggttacaggtgacccgtcacatcttatgtgatcaatgat
aatgtaaaccttccaggtgttagagaatgatgcccttttttgggttaaacaagttacaagtgttcaaggcagaagatacagagacatgtttgggtacata
gctgcacaagaaaaaaaaacaaaaaaacaaaaaaaatcaaaaaacacaatagtatcaccatgatttctgtatgagcttcccaccaagctgggcaacattg
aaagggtagcttcctcaatacaatactattgactgaataaaatcataatgtcctctgcattgatgaccatacagtgctatttgttcattccagttatcaa
atctgagccaagcgccaatgaaagaaaagccatgccaatgttatcttcctgcacaggtacatttgttcacgttgaaagggtccgtccttttagaagcatt
tacgatgcactattgagggcccagactcatcatactcctgcttgctgatccacatctgctggaaggtggacagggaagccaagatggaaccgccaatcca
gacagagtatttacgctcagggggagcaattatcttgattttcatggtgctgggagctagtgcagttatttctttctgcattcaatcagcaattccaggg
tacatttgtggttccaccagagagaacagtatttggcatagaaggtctttacggatatctacatcacactttcataattgaatttgtaagttggtttcat
gaaatgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: