XB-CLONE-4353846
Clone Name: IMAGE:7026418
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783354
[+]
taattaatcccccccccccccagcacacattccagctgctctggggatacttaggaactagcagcacccatccacccatcactgctgttgctgtgctttc
agtctccagtatcctctgcctctacggttttctggcttcctttgcaatcagccttttcaacaaggagctcatagcgatgggacttgctatctttacaaag
caagagccacaaaaagaggaagaaattcccaagctcatggtatcttacaacaattcccgccgttaaaaaccggtttgaagaacaattggctaaaaaaacc
tttaaaaagggaaggaagggggggggaaaattccccctggaaactgttttaatggccctggggacactttccttggaaaggatttcccgaaaacccttta
tcaaacgggggttttatacccccctccccaaaaaaaaaggtccccctcccaaaaaaaaaacccttatctcggggaattctgggagcttttttccaaaccc
ctgttggatataacctcttaagaggggttataatatattctcctcccaccatttttcagcacccctctctgttggttgatatagagggagggcgtctatc
ggaggaacacaatcttgtttaaaaattctcctctcgctgagagggtaattaaaaaataaccgatgcacctaacctatctttcatcttcaacaacattgta
agaaaaaaataatgggcggtctctcacaaaaaagaataatagtaattgatggtgtttgggggaggggggaggaacatattctttttttttttttatatat
taacaaagaggaaggtttgctcgcttcaccnaaaaactctatatacaagctggcccgccacccacgcaccgagagaaataaaacacaatatgtgctgtgt
ggtttttgtgccttcttgtgtatatttcactccgcccccgccgctgctgggagaggaaggtgtnttattttttctttccccaacantaccacattagttg
gttgatgcgcgtggggcgcctgcctcttcctattattttattctgcactgatttcactctatgcggtctcagcacagtcggagaatagggtgaggggcga
atacaacagagaaatctagttgttcctccccctccccccacctccttacacgatcagattacatgaaatggtgatggagacaacaatgcntgcaaggcgg
ccaacggagatatatgataattataatgaggtggtcgatacacatacggcacttttccatctctcatactttattaaaccacgcgtaaatacaagcaccc
ccaccaccagataaggacagcataacagcgggcg
3' EST: CF783353
[+]tttttttttttttttcagcaaaacttgatttggcatatattaattacattgtaattgtgataatatacaattaacaagcagaaacaagtcaagttacgtg
aaacaaattaaaattgtcgtgcaaagtttgttttgaggtaaatgaactcagcactaggcattgttcctgtcaatggcagcagcggctaagaagtaaaggc
atcacacccgtcccctcctggatcaatatgacctataaccctactaatttcagtgcagtgcacttaaggaagaccctaggctaatgccacactcagtgga
ttctctgctagcaacaaaataacactaaggggtgcagttttgtgccccttagctctctaggacttgcatccagggtcaaagtaaatgaccacttacatct
ttgggagaagtccctctgacttaccactccatgaggcccaacgatttgttggaaaacccggggaattgttaccccaaatgaagcatattgctaaagggta
aatctgagactcccctttcctgtagccttaaacagcagaagggaaatttacagaacctttcacaaaggagctggaatttattgaaaaattaaaacaaaag
ggggtaaaaaagcctacagcctggttcaacttttcaagtataaggggggcgaaaataaaaactggggatcaaatttccccaaccccatcaaaaagtcccg
cttaaaaaagattctaaaaacccacccccaaatatccccgcgataacaatacaaacgcggttctccgtgatttaaggggtacccaaggttacaaaacaag
gacgtgtttttctcccagcacaaagaaaaagtagggcatgactgcgcgcatcaaaaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: