XB-CLONE-4353843
Clone Name: IMAGE:7026417
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Gene:
hmgn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783356
[+]
taattaatcccccccccccccggccgactagtctttgtgagtactccatctgcaggagggctcgcacatangaaaaaacaaatatatatatataaataag
tgcaagcaagccagggatttaagtgtgtaggcgtccatttgcagagaaggctgctaagcgttcccgttgcttctgtctttggattcaagcggtgcacaca
agccctcagtgcagccatgcctaagagaaagcaggtgaatgctgctgatgctgcagatgccaaggatgagcccaagaggagatcggcacggttgtcggct
aaaccagccccaccaaaagctgaaccaaagccaaaaaaagacaaggcacccgtaaaggaaaagcctgaggagaaggagaaaaaagttccagcaaagggta
aaaagggagcaaagggcaaacagacagaggaagctaacaaagaagaagctaaggaagaccagccgtctgaaaacggagaaacaaaaagtgacgaggcccc
tgcttctgatggaggcgacaaggaatcaaagtctgaataaccttcatcttgtaccgagtcttttatcagtggttcctgtacctcccttgtacaattcaga
ggaatatttttatcaactattttgtaaatgcaagttttttagtagtttaattctagaaaacacatttttaaaataggagggaaaccccacccattttttt
ggtgtaaaaaaaattttttttttaacacttaatgattgggtaaatttgccccctgtatgggtttgaaaagggcctatgaatactattacccgttaatact
aacaccacaaaactcgtttactggtgggggaggggaaagccctttgaccaatcctaaaaaagggttttgttttataatttggttaaaaaaaaagggtata
gatactcttt
3' EST: CF783355
[+]ttttttttttttttaactttttcaaccaggttttattggtttggtgatcagtacacagacactatgaacaatctgtacattttttttttcctttttaaaa
ctctagtagattggaaatctaggaaaaatatgaaaaaagaaaaaaaataaaaacaatgtcgttgaagtcattggaatccattttctagcggctttccggt
ttttttttttttttttttaaagttcagagagaagttgtttcgtaaaaaatctagcacccctcgtaccagcatctttgatgcaaataattcaacccatttc
tcggccaagaaggagcgaacacgcataggtgccaccttggcaggaaagagatgcaaacacatgaaccgccgtgtcctttttgtttttctccctacttcac
atcacactcactacacactcaaagaagtttccccagaagagattcacagcacttttacaaagttactgaaaataaaataccactacccagaaaaaaaaaa
ataaaatggtaacatcacagatagtacacaattctttagagggaaatccgtcaccttgggagatttattagtttaaaagtgtaaattgtacgcaggacaa
catgagtacaaagaacagttaaaacgttaagacattttaatagccgtgatctttgcgccagttgtaccagtatcgggcactgcctgttcgaccggcagcc
tgcatagagtactagcccgttttgtaacaaaataaaaaaaaacatttttaaactgctcaagggcttgccactccccacagtaaactggttttgtgggtgg
aataataaactg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: