XB-CLONE-4353840
Clone Name: IMAGE:7026416
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Gene:
gcsh
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783358 [+]
taattaatcccccccccccccacctggagctcagcgctcaggaaaatggcgatgcgtgtgctgagcgcgatgagagtcctgagtacagtgacccttgtgc
ggcgtcccgcccaggagtgccgcctggtatggaggtcgtgcttggtcaggagcttggccaccggctcccagttgctctcctctgccttgaaatttacaga
taaacatgaatggatatcagttgaaaatggaattggaacagtaggaatcagcaagtttgcccaggattctttaggcgaagtggtgtattgcagtcttcca
gaagttggcaaaaagctgaataaaatggatgagtttgggactttagagagcgttaaagctgtaagtgaactttactcccccctaacaggagaagttactg
agataaatggagcactaacagacaatccaggcttggtcaatcaatcttgttacgaagaaggttggctaataaagctgacagtagacaacccttcagagct
tgatgatctaatgagtgaagacgcctacgagaaatacgtaaaggcacttgaagactgatcagataattttttggagttgtaacccagctagaaggttttc
cctgtgtangttgagtagtggtgtctcagatcacactggtggggacttcttactgcaacagtaacggttttaatcaaatgcagtttgcataaggactggg
aataagggattcttttttcaagagttggtccctaaaatctttttgcacactatattaattcccgcatttttttttattttttagattttggaataattta
tggctaaaaaccttaagtaaaacctttaatttaaacatggcctggcaataaagtttccagaattccttggtgttaaatttggcctggcaacaattttctt
ccccgcgcacacttggaagagagttttatttgggaaacaatcgcgcgggttaaa
3' EST: CF783357 [+]tttttttttttttttgttaaaggtctcatatcagttttaattacaaggcattatttctgaacgtgcaacaaactttgtcttcagtcatggctgttggtac
caaaaaagggatggttttcattccagcacacaaggaagaagtctttaaagaaatcactacattaccggatgttcaataaactcttcagttgctggagaac
tctgcagcaattacacaagaatctgaacttattgcagcatgttaattaaggtttactaagttttagcataattattcatatctaaaaataaaaaaatgcg
gaattatatagtgtgcaaaagatttagggacaactcttgaaaaagaatcccttattccagtccttatgcaaactgcatttgattaaaaccgttactgttg
cagtaagaagtccccaccagtgtgatctgagacaccactactcaacctacacagggaaaaccttctagctgggttacaactccaaaaaattatctgatca
gtcttcaagtgcctttacgtatttctcgtaggcgtcttcactcattagatcatccagctctgaagggttgtctactgtcagctttattagccaaccttct
tcctaacaagattgattgacccagccctggatgtctgttaatgctccatttatctcaataaattctcctgttaagggggaataaagtttacttacagctt
ttaagcctctctaaagtcccaaaatcatccatttttattcagcctttttgccaaccttccgggaaaaatgccataacaccccctttcgccctaaagaaat
ccg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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