XB-CLONE-4353837
Clone Name: IMAGE:7026415
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Gene:
eef1b2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783360
[+]
taattaatcccccccccccccgcttttctttacccgtgtgtgctaggcttcttgagtgaattgctgtaatgggtttcggcgatctcaaatcccctgcagg
ccttaaggtcctgaatgagttcctggccgacaagagctacatcgagggatatgtcccttcccaggctgatgttgcagtatttgatgccctctctggtgcg
ccccctgctgaccttttccatgctctgcgttggtacaaccacatcaagtcttatgaaaagcagaaaacctgcctggtgtgaagaagcctctgggaaacta
tggccctgttaacatagaagatactacaggcggtacagcaaaagataccaaggaagaggatgatgatgatgacattgacttgtttggttcagatgatgaa
gaggaaaatgaagaatcaaagagggtccgcgaagaacgcttagcacagtatgaagcaaagaagtctaaaaaaccagcacttattgccaaatcatccattc
ttcttgatgtgaagccatgggatgatgaaacggatatggccaagctagaagagtgtgtcagaagtatacagatggaaggcttggtgtggggagcatcaaa
gcttgttcctgttggatatggcattaaaaagctgcagatccagtgtgtggttgaagatgaacaagttggcacagatgttttagaggagaacatcactgca
tttgaagactttgtgcaatccctggaagttgctgctttccacaagatctaaactgatttcatgaaacccccatacttttacagcactttcaaataaaaac
atttggcctaaatggtgaagaaaaaaaaaaaaaaaaagggatccccccagtttccaaaagcgggccgcccaccgcgggggggaaacttgggcccggtggc
cccaaccttgta
3' EST: CF783359
[+]tttttttttttttttcttcacattaagcaaatgtttttatttgaagtgctgtaaaagtatgggggcttcatgaaatcagtttagatcttgttgaaagcag
caacatccatggattgcacaaagtcttcaaatgcagtgatgttctcctctaaaacatctgtgccaactttgtcatcttcaaccacacactggatctgcag
ctttttaatgccatatccaacaggaacaagctttgatgctccccacaccaagccttccatctgtatacttctgacacactcttctagcttggccatatcc
gtttcatcatcccatggcttcacatcaagaagaatggatgatttggcaataagtgctggttttttagacttctttgcttcatactgtgctaagcgttctt
cgcggaccctctttgattcttcattttcctcttcatcatctgaaccaaacaagtcaatgtcatcatcatcatcctcttccttggtatcttttgctgtacc
gcctgtagtatcttctatgttaacagggccatagtttcccagaggcttcttcacaccaggcaggttttctgcttttcataagacttgatgtggttgtacc
aacgcagagcatgggaaaaggtcagcaggggggcgcacccaaaaagggcattcaaatactgcaacatcagccctggggaagggaaaaatcccctcgaagg
aaccttcttgtccggccagggaacctcatttcaggaaccttaaggggcctggcagggggaattttgaaaaaccgcccgaaaaaccccatttaacaagcca
c
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: