XB-CLONE-4353834
Clone Name: IMAGE:7026414
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Gene:
eif1b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783362
[+]
taattaatcccccccccccccggtgactctggaagagcaagtgacgcctaagctgtgcttaagcttgtggggctgttctatcactaaaccagcacacatt
agctgccttgtagtccattgtgagacagcgcgatagagcagctttgcctccttgcctagaaaccagcgcctgacgaacatcgccgtagagtcccacagct
ttactgtcttctgctccgggccagcgccagccttttcctctgcacaccgtgctcgtaataatatcgcatgtcctctatccagaacctcaactctttcgac
ccctttgctgatgcaactaagggtgacgacttactcccggcagggactgaggattatatccatataagaatccagcaacgtaacggcaggaagacattaa
caactgtccagggaattgcagatgattacgacaaaaagaagcttgtaaaagctttcaagaagaaatttgcttgtaatggtactgtgattgaacatcctga
gtacggagaagtcattcagcttcagggggatcaacgaaaaaatatctgccagtttcttatggagattggcattgtcaaggaagaacaattaaaggttcat
ggtttctgagatgtggattacataagattcttgcgacttgccaatcttggctattttgtgaaccatgcatatttgcagtagaaggacttttaacatgtct
gagcattcaaaactaggtcccttttttgcatgactaccgggaaaagtgtaatgattaggctagtaacacataaaaaaaatagctggtagatggtaaaaaa
aacagtgtggtttaaccacatttcccatgggaaatgtttttagtgctgggggtcctttttn
3' EST: CF783361
[+]tttttttttttttttcttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttcgcc
cccccccctttggtttttaacccccaaaaacttttttcccctcccgggggggggggggggaaaaaaaaaaaaaaaacccccccccccaaaacttttccct
tggaaaggggggaaaaacccctttttttttccccccccaacccttttttttttttggggtacacccccaaaaaaaccctttttccgggggccccccaaaa
aaagcccctttttttaagccccccccatttaaaaaccccccccccccaaaaaatgggggggtcccaaaaaaccccaaattggccagcccccaaaattttt
tttttcccccccccccaaacccccgaaccctttttttttttcccctgcgggggccccccccccaaaaaaacggggcaaatttttttttggtcccccccca
aaaaaagaaaccctcccccccccccgggggtttccccccgccccccatttgaccaaatttttcttcaaaacattttcaccccttcttttttggtaaaaac
cccccgaatcccccggaaagttgttatatgctcccccccccctaagtcgggcgggagatttttattaggggaaaaaatcccccacccccccggggaggga
aaaccccccccccccctatttttccccccacaaagggggcccaacaaaaatttgtgtctctcctcgaaacaaccacccccccattattttctcccccccc
ccgtgtgtcaaaaaaaaaaaaacgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: