XB-CLONE-4353831
Clone Name: IMAGE:7026413
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Gene:
XB5774338
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783364 [+]
taattaatcccccccccccccaggtactgcaggggaaggagaaagaagccggacattaacccaacccactgacaggatgaagtgcattgtggtcctgctg
gtttgcatctccattgggtgggtccattcctgcaacgtcctgcccggagcaattaatttggcaaagtcgggggacgccaagcaaagctccacgtatgccc
ctcggtacagcgcccaagcagcggccgatggcaatagggcaaccaatctcctcacagccccctgtgccagcactgcttatgacaatcccgcttggtggca
ggtggacctgaagaagacctacagcgttgacactgtggtgatagtgaacagaggggactgctgccgagagcgcctgagaggagctgagattcgcgttgga
aattccccagataataacaacccagtatgcggcatcatcaacgacttctcaaacctcattaccactctgtgctgtgacgggaaggagggccgctatgtga
gtgtggtgattcccgggcggaatgaatatctcaatctctgtgaattggaggtttatgggcaggaagttaagctgtggcaggtaatgtggcattattggga
gtggcccccacaaactccaattacagatccgagtatggccgcccgtaaccctaatgacgggcacaagaacgcaaatatgatgaaaggcttcgtgttccct
caccggtagccgacaacccgggcttgggtggcagctgggacctgaaaaagcccttacctcgtgggaaaaaagtggtttataatggaaaataaggggaact
gtttgcttgggaaaccttccgccaagggggcccaaaggtcccccttttggaaatttccaacctaaaaatataaaaaccccccctttggtaggccaccctt
taacaatgaaacaacttccccaa
3' EST: CF783363 [+]tttttttttttttttcatgaattcatatcaatttattcatcgtctgtttattgtgattaattctaaaaggttgagttttcctttttataataaatgtgag
acattccaggaaatgagtttttgttcagattttcgcataaacctccacttcacagagctggagagtctccgccctcccgggaatcaccacgctcacgtag
cggccccccatcccgttacaggacagggcgatggtcggtttagagacgtcggtgacaacgccgcatactgggttgtcacgggatgatgaactcccaacgc
ggacctcggcccccaacaggcgctccccgcagcagtcccctctgttcactatcagcactttctccacgcggtacggcttcttcaggtccagctgccacca
agccgggttgtcattaccggtgtgggaacatgatcccgccatcatatttgagttggtattaccatcgatggcggtggcggcgttgtacatggggccgtaa
gttgagctctgtgtggcttctcccgatccggccacgttcactgcagcttctaccccataaacctccacttcacagagctggagagtttccgccctcctgg
gaatcaccacgctcacgtagcggccctccatcccgttacaggacagggtgatggtcccttgggaggtgtcagttacggtgccccatactgggttcttatt
attggttgaacttccaacgcggacctcggcccccttggaaaagccccccagcagcaagtccccccctgttcactaatccaccactttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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