XB-CLONE-4353817
Clone Name: IMAGE:7026322
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Gene:
eef2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783373 [+]
taattaatccccccccccccccgctcttccgcctgtttgtctgctgtgaggcagactgtccgtatcggcctaagcccgtcccgtgaagagaatccaccgc
catctgccacaatggtgaacttcacggtagaccagattcgttccatcatggacaagaaggcgaacattcgtaatatgtcggtcattgcccatgtggacca
cggtaaatctactctgactgactcactggtatgcaaggctggtatcattgcttctgctagggctggtgagactcgttttacagataccagaaaagatgaa
caagagagatgtatcaccatcaaatcaacagcaatctctctcttctatgagctctctgagaacgacttggcttttatcaagcaaagcaaagatgggtgtg
gcttccttataaacttgattgactcccctggccatgtagacttttcctctgaagtaactgctgctcttcgtgtcacagatggtgccttagttgtagtgga
ctgtgtttcaggcgtctgtgtgcagactgaaaccgtgcttcgtcaagccattgcagagcgcatccgccctgtgttgatgatgaacaaaatggatcgtgct
cttttggagctgcagttggaaacagaagagctctaccaaacttttcagcgcattgtgggagatgtttatgtgattatttccacccttacggtgaaaggag
agactggaccccatgggaaaatatccatgattggaccctgtttcttggcacagtttggtttttgggctctggttctgcatgggctggggcttttcacaac
ataaaacagttttgccagaagattgtattgtcgccaaaattt
3' EST: CF783372 [+]atacaagctggcagaaggttctaggaagttttttgccatcagcgtttgttgcagacttgctgaactttccatttgcagggtcaaaatacctatcacccca
cagcttcttcatcatgtcctccacttttttggcccgttctgcaggagccaactgaccttccccctttgctgcaaattttgcgacatacatctctgcaaac
tgttttagtgtgaaagcccagccatgcagaccagagccaaaaccaactgtgccaagaacagggtcaatcatgatatttcccatgggtccagtctctcctt
caccgtaggtggaaataatcacattaacattctccacaatgcgctgaaaagtttggtagagctcctctggttccaactgcagctccaaaagagcacgatc
cattttgttcatcatcaacacagggcggatgcgctctgcaatggcttgacgaagcacggtttcagtctgcacacagacgcctgaaaacacagtccactac
aactaaagcaccatctgtgacacgaagagcagcagttacttcagaagaaaaagtctacatggccagggggaatcaaatccagttttataaggaaagccca
ccaccccatccttgcctttgcctggataaaaagcccaaattcggtttctcagaagaacctcctttgnaagaaaaggagaattggcctggttgaattttga
agggggggaaacaaaccccccccttggttccaacctttttcggggggaacctgggaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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