XB-CLONE-4353803
Clone Name: IMAGE:7026316
|
Gene:
lsm12
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF783382 [+]
taattaatccccccccccccgacactcttgactgcctctgttaggaactgtgacgcttgtgagggctgcctctctgatcataccacaaaatgtctgacaa
accagatatggctgagattgagaaatttgataagaccaagctgaagaagacagaaacacaagaaaagaacccactcccatcaaaagaaacgattgagcaa
gagaaagcaacttcataatgatcccccatcacccagatatgcactgtacattccacaagcattgccttcttattttacttttagctgtttaactttgtaa
gaaataactcaaaatacaaagaatttggagaacatgttctgtgctgccctgttacaccaaaagaaaggaataagaactactgaacacggaatgaaggcgc
tgcctttccatctgcctgtctggctggctttggttctggtggcattgaaagatctttcttgattatttaaggagaaccagtgtggaaataacagaactaa
agtaacatgctctgatgtgggtgggatgaaagctgcaccaaggtccagcgagctgtaaaatacggtcaaatcgagtgccattttatttgttcaaaattaa
ttgaattattggaatgcacaaatttttttttaatatgcaaaataaaaatgataaaacatttaaaaaaaaaaaaaaaaggatccactaatttctagaagcg
gccgccacccgcggttgggagcttggcccgtggccccaactttgtttatttgcagcctttataaatggtttaccaaataaaagccaattacccatcacca
aaatttttcacaaaataaaagccattttttttttcacctggcattctcttaggtta
3' EST: CF783381 [+]tttttttttttttttaatgttttatcatttttatttgcatattaaaaaaaatttgtgcattccaataattcaattaattttgaacaaataaaatggcact
cgatttgaccgtattttacagctcgctggaccttggtgcagctttcatcccacccacatcagagcatgttactttagttctgttatttccacactggttc
tccttaaataatcaagaaagatctttcaatgccaccagaaccaaagccagccagacaggcagatggaaaggcagcgccttcattccgtgttcagtagttc
ttattcctttcttttggtgtaacagggcagcacagaacatgttctccaaattctttgtattttgagttatttcttacaaagttaaacagctaaaagtaaa
ataagaaggcaatgcttgtggaatgtacagtgcatatctgggtgatgggggatcattatgaagttgctttctcttgctcaatcgtttcttttgatgggag
tgggttcttttcttgtgtttctgtcttcttcagcttggtcttatcaaatttctcaatctcagccatatctggtttgtcagacattttgtggtatgatcag
agaggcagccctcacaagcgtcacagttcctaacagaggcagtcaagagtgtcggggggggggggggaattaattaaactcgagtgccaaaaagaaccaa
gtagccttgtattcggggcccggataaaaacgaaggtgccagtttgggacctggggaatggaacacc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: