XB-CLONE-4353797
Clone Name: IMAGE:7026309
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Gene:
rap1a
,
ptbp1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783386 [+]
taattaatcccccccccccccccacagttcccggaggcactggcggggattagcgctaaagggaaaagagccggctctgctcaggctgccagaggtgcaa
catgtccgggaaagtgaaggtgcgagcggcggccaatgccgaggccatgtcgggggtcgtgtctgtaaatgaacggatcttgcgggactgtcactccctg
tacagcgaccccgaaaccggtctcatcgctctcgcctcccacctgggactctcttttctccccccccgcaagaagatctttgtgatgctgatgggaaatc
actcggccgggaagagcagcttcattaactggtatatcgaggagcacatacagaggacgggatttgccatcgagactcatggatttgcctttataaccag
cggccgcaagagggaatcgctgactggtgatgccacgatgcacttgtatcctcacttcaagcccttacaaacctttgagggcatgtccgagcacctctca
acggaaatttcccccctcgtgccaaaaacaagttcagcctgggtgacatttcgtggattcctccggggcctggtggtactggggcaccttgaaatacccc
ccttttgaccttttcccaaaagccactttcttgttgacctcccggggtaacccggcgctgaaccttggagggattggggggtttctttattccccctaaa
gtgggtccacggtcccatactgtctaaacagaaacgttctgggctattttcccggggaattaaaaacctcctccaatatcctttccctgccgggagttac
aaccagccatttctcctaatctctgatgccccctgtcccctaatcttatagcccctccaccaccatatatattttgccacacttcacccgggtagagctt
cttgac
3' EST: CF783385 [+]tttttttttttttttctttttttttttttttttttttgtcaaaaaataatttatttggtgtaaaaaagcaaaaacataagatcagagactgctacatggc
aacaccctccccggaccccccaatactggcagtgttaggtttcacagttggttctatttattatgaacaattacatgtaaaaaggcactaggagatctat
caggggagccactgcccagaccgtgcccacagcgaggtgctcagtatcactatacacggccgccagtccctttcattgcaataatgtttgtggctgatgg
gaaggggggttcggatccatgccggttctgtctctcagataatttgggactcactgtaacaaatacacagtagggccctgctgctgcgcccgatagaatg
ttccgataaaaatggatgcccaatctttatggggaggggagtagagcacccgcccgttgcccagctgaagtctaatttgtatggggaggggaatataata
tccgcgggttgcccagcctgaagcccactcctgtatgtggaaggggagataaagtaacccccgggtttgccccccctgatagccctattttgttcatttg
cagtccccactacgcccttaaaggttggccaaaacctcgagaaaaaaaaattcaagacgcccgggaggggcaattaataaataagctcgccggggtttga
accaaatttaaatactccccccccctggatcgaagtgaaaggcaggaattaaaactttacccctccgcgcctattctctaaagcattatattccccaccc
tattgataacaaaatataaagtggaaaccttacacgaaaacattctcttgtatatcccccctccgctctaatatctattataacactaatcccaccccgc
gtctagtgcacacacccacacaccgtattttataaataaaaaaacccccgcctgcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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