XB-CLONE-4353785
Clone Name: IMAGE:7026305
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Gene:
cdca8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783394 [+]
taattaatccccccccccccaacccccnngaggtttgaatctgtactgtggccggtgaggcgcttccttggaaagcgaaggatttcaccttttcatcatg
gcacccacgaaaaagaaaaccagtcgcaaaccaaaaaatcgttgtgtgaaaaatgagaaattggcttcattcatcaaggattttgacagccaagtaaaga
ttatcacagaagaattgaaggcaagtgttgtgaatatcctcacagaagtggacagccagtacaatattgaaataatcacactacctatggctcataagag
aaatgtgctggctcgattacattgcaaaaggtggaagtcaaaaggctttggaagcggctgctactgtgaaggtagacatggaagaaattacatctactgc
ttccaaaacatcattcaagctggataaaaacagtgaaaaagggaaagtgtaagtctgatgagacattggaaccaaatcctctgcacaatggaataagaac
tcaaacaaaggccacagttgctgttaagaaaccatctactgcaagaaaaactcgagcctccattgcaaccctgactaacaccaccaaatggacaagttta
caaaagttctgccaccaccctttttcaacccaataaaatgacgaccccctttactggggaaacataattcggctgcaccctccctttacatagcatttcc
aggcataattttatcactcgccccgaaattgacgctctaccaaaggctttggccgcggaagtaccccataccttcctcttagtttcggcttcacccgggt
aaggctccctttttcgccccggctccttcgcatttcaaccctttcccctcatcctaccatttccacc
3' EST: CF783393 [+]tttttttttttttttaaactcttaaatcatgtgtgcgtgttttattaataaaaaaaaacactgttagaatatcaagataaagcagtaacatcagagtaat
gggtttaaatgttttccctgccacttcgcattctatacaccaagagtgtcaaatgtgctacacaaaagctgttgtgaacagcaacttttcagaggagctg
cagcccataacaaaggttttgtatttaacaactctctacaaagaaataaaatacacagcttttacagttatttacaatgatacacagaaatgtatctaag
ataaaaaaacaaaattacaagatggacgaatgaagaattcactttagtttcttgcagaacctttggagttgacttgagaggagtttaatgttttcaaacg
cttgattgtctaagcgattaatatccaaactgtccacctcactggccagaagacggatattctttccatctcctgcaggcacattgataaacagctcatc
cataccagccaaagggctgccgttagcagaaaatgtgtagactggttcctgcaagcatggcgtacgcagtgctggggttttaaatatgctggtatcaatt
ctaggggtagcagcaggtgtgtatcccagtaaagaggtctcaatttgtttgcttgcagagggtgttgcacggcctctcttacttgtccttttgctggtgt
tagtcaagtttgcagtggaagctcgagtttttcttgcagtagatggtttctttgcagcaactttggccttttggtttagttcttantacactttgcagaa
ggatttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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