XB-CLONE-4353780
Clone Name: IMAGE:7026303
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Gene:
nabp2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080502
[+]
GGCTCATTGTGTCAGTGTTCGGGCGCCTATAGGTCAGACCAGCTCGACTACTTCCTGTAAGCGGCGAGAAGCTAGTGTGGAGTGTGTGCGGATAAGGCTG
CAGTACCTCTCGGCTGGGGATGCTGTGCGGGTCGAGCTAGCGTTCTGTCTATAGCAAGATTACAATAAACTGAGCACGACACTGAAACAACTGTCATTAT
GACCACAGAAACCTTTGTGAAAGATGTGAAGCCTGGACTGAAAAACTTAAGTGTTCTATTTATAGTCCTGGAAACAGGAAGAGTAACGAAGACTAAAGAT
GGCCATGAAGTGAGAACATGTAAAGTGGCTGACAAAACTGGTAGTATTAACATCTCTGTTTGGGATGACTTGGGGAACTTTATTCAGCCGGGAGACATAA
TCAGGCTCACTAAAGGATATGCTTCTTTATTCAAGGGCTGCCTGACTCTCTACACAGGAAGGGGAGGTGATCTACAGAAGATTGGAGAATTCTGTATGGT
TTATTCCGAAGTGCCCAATTTTAGTGAACCCAACCCAGAATATATTGCCCAGCAGTCCCAAAACAAGCAGGCTCAGGCAGAGAGTGGCACTGGGACAAAC
AGTCACAATTCATCCAGTCCAGCTCCTCCAGCATCTGATCTAGAAAATGGCAATGGATCGAACAGCTCTGGGCCTCCTACCCATCAATCCACAGCACCTA
CTCACTCAACCAGCGGGCGGATTACACGCAGCCAGCCTAACCATTCAATTCCCGGAGCTCCAAACTCTGTCAGTAATGGCAAAGAGCCACGGCGCACAGG
GAAAAGATAGCAGCCATAGCCTGTTCCTCATCTGATTAGAGAATTCTCCTCCCTTCGCCCAAAGCACATATGCCAAGTGTGAAGGGGGAATTGGGATGAT
GTTACTTACTGACTTTTATAAAAGAATAGTCCAGGCATCTGTTTTGCACTAGTAGAAAAGACATTGAGTGAACGCAAGCCAAATCAAGGGTGCGACGTGG
AGTATATTGGCCAATTCAAACATGTTTTCCGAACGCCTTATTGTATTTAGACAGATTTTAGGAGCTTCTTGTTGTTAAAACTTTAAGATCTGAAATTATT
TTATACACTGCTTTTTTTCCACTCCCAAATGAAGGTGGAATCGGGACTTTTACTAAAAGACTTCACATTTACACTGCAACGGTTTCTTATGGCAAAAACA
GATTCAATCATGTAATATACTCGTATACCATATGCGTGTAACCCCCTGCAAAATGAAAATGTGGTATTGCCTTTTAGAATTATACACTAGGCTACTGGCA
TTTGCTTTGTTTACAATGCCAGTTGTTAAGATACCAGTACAAAAGCAATATTGTGCAGAAGAAAAGCGTCCCAGTGAATTCAACATTGCCAGTAGGCTGA
TACTTCTGCTGTTACTCTAAATGATACAATAGAACCATTAAAAATACAAAGCCACCCTAAAAAGCCAGTGGTTTTTATTTGTCAGAATGATGTTTTAGCA
GGTAAATTTGAATCTTTCTTACAATACTGAATAAGGCCTTACTGTTAGAGAAGCAGCATAGACATAGACCATTCCAATATAAAGTGCTTAGTTTTGCCTG
GTTTCCTGCAGCGTGTTAGGGATCAGAGGTGCCATGCAGTGATCAGGACAAATAAGGATCCCTTCCCTGTGTTCTGTGAACATAAAGGTTGCTTTTAATG
TTGCCAGGCTTTAATGGAAGGGATTGAGTATGTATCATTTTTACACTAAATTTAGGTTTTCCATCTCTGCCAAAAGAAAGTGCCATCTGTACTGTTGTAA
GTGCTTTAAATTTGTATAAATTTGCACCACTAACCCAATAATAAGGCATTAATTTGGTAAAAATGAAATCGGAATATGGCTAATAACATGCAGGGTAGTA
GAGAAAAAAAACAAAGAGAAAAAAAGAATAAAAAAAAAAAAAAAA
5' EST: CF783397
[+]taattaatcccccccccccccggctcattgtgtcagtgttcgggcgcctataggtcagaccagctcgactacttcctgtaagcggcgagaagctagtgtg
gagtgtgtgcggataaggctgcagtacctctcggctggggatgctgtgcgggtcgagctagcgttctgtctatagcaagattacaataaactgagcacga
cactgaaacaactgtcattatgaccacagaaacctttgtgaaagatgtgaagcctggactgaaaaacttaagtgttctatttatagtcctggaaacagga
agagtaacgaagaccaaagatggccatgaactgagaacatgtaaagtggctgacaaaactggtagtactaacatctctgtttgggatgacttggggaact
ctattcagccgggagacacaatcaggctcatcacgggatatgctctctttattcaagggctgcctgactctctacccaagaaggggagatgatctacaga
acattggagaattccgtctggctttttccaaagtgcccaattttagtgaaaccatgccagaatatattgccccatcaatctcctaaacccacctggctcc
gggtaacagagtttcccaccggccaaaaactttttcccctcttgttcccccccccgcctaccggcatattagcgcggatctttcggagagggcctttggc
ggttccaaaaccctttcacggtggtcttgcccattccagcgcaatttcaaaagcccatccaaatattttccaaccctgcgttttccccgcctttccccgt
aatgccttgccccctatccggtttctctttctccccgccgcactcctctttcgcccttttctcccgtgttctttggtcc
3' EST: CF783396
[+]tttttttttttttttattctttttttctctttgttttttttctctactaccctgcatgttattagccatattccgatttcatttttaccaaattaatgcc
ttattattgggttagtggtgcaaatttatacaaatttaaagcacttacaacagtacagatggcactttcttttggcagagatggaaaacctaaatttagt
gtaaaaatgatacatactcaatcccttccattaaagcctggcaacattaaaagcaacctttatgttcacagaacacagggaagggatccttatttgtcct
gatcactgcatggcacctctgatccctaacacgctgcaggaaaccaggcaaaactaagcactttatattggaatggtctatgtctatgctgcttctctaa
cagtaaggccttattcagtattgtaagaaagattcaaatttacctgctaaaacatcattctgacaaataaaaaccactggctttttagggtggctttgta
tttttaatggttctattgtatcatttagagtaacagcagaagtatcagcctactggcaatgttgaattcactgggacgcttttcttctgcacaatattgc
cttttgtactggtatcttaacaactggcattgtaaacaaagcaaatgccagtagcctaatgtataattctaaaaggcaataaccacatttttcattttgc
cgggggttacccccatatggtatacgaaccatattacatggattgaatcctgttttctgccaataaaaaacccggttgccagtggtaaattgttgaaagt
tccttttaagttaaaaaaattcccgcaatttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: