XB-CLONE-4353759
Clone Name: IMAGE:7026295
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Gene:
ctbs
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783410 [+]
taattaatcccccccccccccccgctgctgcttgtaggaaggtagcccaggaggcagatcatggcagaactaagtgcaggctcttgggtcttgtggccac
tttttattatgctctgtgtgtccctgtgtgctgctggctgcccctgtacagaccccaggctctgtgagccaatcaccgacagccgggattttgaggttct
cgtgttctacacaaaaggaaaaaactggaagttctatgactggtctcaggttaccaccatcgcattgtttgggaagtatgacccagaacttctgtgtttt
gcacattcaaagggagcaaggcttgtattaaaaggcgatgttccactcccatacatcgtggatctaaaaaacacaatatcttggataacatacaatgtgg
agttggcaaacagtcagtttatggacggaattaatctggatatagagcagtctgtgctaaaaggttccccagagtattatgccttaactgctctagcgga
agaaacaactgaggctttccacagaaaaattccctggttctcaagacaacttttgatggggccatggttcccccgaaccggtttggacgaaaaatgtgtc
tttaaatctaccccaggggatacgcttaaaattcttgccggacttccctttttccgttaaggccccccttttacccagccaatccctatgatattggacc
caaaattgttgtcgggctaacacactaactttttccctacccttacaaaaaaccttttacccccgctttccccagtgaaagtctcgcccttccaacaggt
gtacctttgcgatggtttaaccaaacctttcccatataagggccttgtcccctttttcggattccgtctccccccaccaccccctcgtcttccccgcgta
actcgcctaacggtatcctcc
3' EST: CF783409 [+]ttttttttttttttatataaaaactattttttattaagtatatttttatggcccgattctccatattagtaatttcttaatttacaagagccccaccccc
tttaaggcattccacatgtctttagtttgagtctcagctatcggatcctgtgagtaatctagtagatctccgttccacatgcctatgcctcttaatccaa
gtttcttgatgtacgcagatttcagggagatgctcactggatcatcataccaaacttgatggaactgcccctttgcatccttgtaattataaaaaggaga
tttctgtacatcatcccatagccttccagtcagagaactgttgacctgcttcgtgatcttactgtatgggatctgcttccctgctgcatcactgcaaggt
gctccccgaaaagggacctccttaagagtacaattattatcctctaagtccagacacgggtagtcgtaaccataccaagggacacccattaccagcttct
tgggatcaatgtccagttgagtgaacttctgataacctgataatgttttattgagaggagaattggctgatgccacacactctgtccatatctgtgattg
ctcgtcataggacataacgaaaaggaaatcacatgatttccgctatcccctggtgtattgtagcaccctttcatcccccacaggccgggaagaccattgc
ccaccatcaaaagtttaccctgtagaggccaggggaatttcctctggtggaaaagccttccagntagttttccttcccccttagaaggcaggtttaattg
caataattaacttcttggcgcgaaacccttttgtaaacaacttgaacttgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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