XB-CLONE-4353745
Clone Name: IMAGE:7026290
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Gene:
amy2b
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080454
[+]
GTCAGGTGATCTCTGAGGGAGCACACAGCCCATCACAAAATGGTGTCTCAAGAGCAATGATCCAGGTGCCACTGTCTGCTCCTCCTCCCTTCCATGGAAT
ATAAAATGAGAACTGGGACACACAAGGATTGCAAAATGAAGCTGTTGTTTTTCCTGGTGGTTATCAAACTTTGCTCTGGACAGTATGATCCGAATGTTAA
GCCTGGAAAAACGTCTATTGTCCATCTGTTTGAATGGAAATGGGAAGACATTGCTGATGAATGTGAGCGATATTTGGGACCCAATGGATTTGGAGGTGTC
CAGATCTCTCCACCAAATGAAAATCTGGTGATTACTAAGCCCTGGAGACCATGGGATGAAAGATACCAGCCTGTCAGCTACCAGCTTTGCACACGTTCTG
GCAATGAAGAGCAGTTCATAGACATGGTGACCAGGTGCAACAATGCTGGGGTGCACATTTATGTGGACACAGTAATAAATCATATGACTGGATATGGGGC
TGGGATTGGAAACAGTTCAACATGTGGGAGCTACTTCAATTCAGGAGCAAGAGACTTTCCAGCTGTCCCATACTCATTCCTGGATTTTAGTAACTACAAA
TGCAAATCCCACAGTGGCGGTATAGAAAACTACAATGACCCCTATCAGGTCCGAGATTGTCAGCTCCTTGGTCTCCTGGATCTCGCTTTGGAAAAGGATT
ATGTTCGTGAAAAGATCGCTGAATACATGAATAATCTGATTAATATAGGAGTGGCTGGATTTCGTCTCGATGCCTCTAAGCACATGTGGCCTGGAGATAT
CAAAGCCATTCTAAACAGGCTTGAGGACCTGAATACCACTTGGTTTTCTAATGGCACTCGGCCTTTTGTTTATCAAGAGGTGATTGACCTGGGAGGAGAG
GCCATTTCAGCAAGTGACTATTTCGGGACTGGTCGGGTTACAGAATTCAAGTATGGAGCCAAACTTGGAACAGTCATTCGTAGATGGAATGGAGAAAAGA
TGGCCTACCTGCGGAACTGGGGTGAAGGCTGGGGGTTTATGCCCAGTGACAGAGCCTTGGTATTTGTGGATAATCATGACAATCAGAGAGGTCATGGTGC
TGGGGGATCGTCAATACTTACGTTTTTTGAATCTCAGCTGTACAAAATGGCAGTGGGCTTTATGCACGCACATCCTTATGGCTTTACACGCGTGATGTCA
AGTTATCGCTGGCCTCGACATTTTGCAGATGGAAAGGATATAAACAATTGGATGGGGCCTCCGAGCAATCCTGACGGATCCACCCAACCTGTCACTATTA
AAGCTGATACAACCTGTGGTAACAACTGGGTGTGTGAGCACAGATGGCGCCAGATAAAGAACATGGTTATATTCCGGAATGTGGTTGATGGCCAGGCCCT
CACTAACTGGTGGGACAATGGAAATAACCAGATTGCTTTTGGGCGTGGTAATAAAGGGTTTATAGTCTTCAATAATGATGACTGGGACCTGGATGTGACT
CTGAATACTGGCCTGCCAGCTGGAACCTACTGTGATGTCATTTCAGGGCAGAAACAAGACACAATGTGCTCTGGCAAGAAGATCACTGTGAATAAGGACA
GCACTGCTCACTTTCAGATCAGTAACACTGCAGAAGATCCATTTGTTGCTATCCATGTAAATGCCAAGTTATGAGCTTGACATCATTCTAATGAATTTTT
AAATAAAGTGATTAAACAAAAAAAAAAAAAAAA
5' EST: CF783419
[+]taattaatcccccccccccccgtcaggtgatctctgagggagcacacagcccatcacaaaatggtgtctcaagagcaatgatccaggtgccactgtctgc
tcctcctcccttccatggaatataaaatgagaactgggacacacaaggattgcaaaatgaagctgttgtttttcctggtggttatcaaactttgctctgg
acagtatgatccgaatgttaagcctggaaaaacgtctattgtccatctgtttgaatggaaatgggaagacattgctgatgaatgtgagcgatatttggga
tccaatggatttggaggtgtccagatctctccaccaaatgaaaatctggtgattactaaaccctggagaccatgggatgaaagacaccagcctgtcagct
accaactttgcacacgttctggcaataaagagcacctcatagacctggtgaccagacgccatactgctccggtgcacacttatctgcacccctctctttc
tccctattactggacatggggcctgggaattgtaaaaccctctttaccctttccgtaacctacctttcaatctctatgcacccagtattacccactctcg
atcctcgcccctttacctcccgccctctgcgatttttcagtttatttaccaccatggctttttccctcacacacgggcgaggtattggataaaatcttaa
taatcgcctttctccccccccgtttttaaaaatctggtcacgcatccttttacgaccccccgtgtgaaattgtttaccccccctgaagtccgaccctgcc
gtctctcaaacctttttccctatgaacacgacctcctgaaccaattgcattctgcactgttgatatatccgtagcaaccgcttggatcctttgtttcccc
acccaccccctccatatttccccacctcgcgccc
3' EST: CF783418
[+]tttttttttttttttgtttaatcactttatttaaaaattcattagaatgatgtcaagctcataacttggcatttacatggatagcaacaaatggatcttc
tgcagtgttactgatctgaaagtgagcagtgctgtccttattcacagtgatcttcttgccagagcacattgtgtcttgtttctgccctgaaatgacatca
cagtaggttccagctggcaggccagtattcagagtcacatccaggtcccagtcatcattattgaagactataaaccctttattaccacgcccaaaagcaa
tctggttatttccattgtcccaccagttagtgagggcctggccatcaaccacattccggaatataaccatgttctttatctggcgccatctgtgctcaca
cacccagttgttaccacaggttgtatcagctttaatagtgacaggttgggtggatccgtcaggattgctcggaggccccatccaattgtttatatccttt
ccatctgcaaaatgtcgaggccagcgataacttgacatcacgcgtgtaaagccataaggatgtgcgtgcataaagcccactgccattttgtacagctgag
attcanaanacgtaagtattgacgatcccccagcaccatgacctctctgattgtcatgattatccacaaataccaaggctctgtcactggggcataaacc
cccagcctttcacccagttnccgcaggtaggccatctttttctccattccatctacgaaatgactgtttccagttttggctcccatacttgaaattctgt
aaacccgaacagntccggaaatagttcacttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: