XB-CLONE-4353740
Clone Name: IMAGE:7026286
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783422
[+]
taattaatcccccccccccccgagcagtaacatacaacaccgtccttccgctcggtcagtcaaactttgcaaccatgtgtgacgatgaggagaccaccgc
tcttgtctgtgacaatggctctgggctggtgaaagctggctttgcaggagatgatgctcccagggcagtgttcccatctattgtgggtcgcccacgtcac
cagggagtcatggtaggcatgggacagaaggactcctatgttggggatgaagcccaaagcaagaggggtatcctcaccctgaagtatcccattgagcatg
gcataatcactaactgggacgacatggaaaagatctggcatcacaccttctataatgaacttcgtgttgcccctgaatagcaccttacactgctaacaga
atcttcgcttacccctaaagccaacagaccaacagatgacacaaatcatgtttcgaaaccttacaatgtagccctttcatgtatgtttttaaatccaaag
gccgaagactgcttacttttttggcccccttggtctgttaaccacctgggtaatttgctgccttgtgaatctctcgtcgaatattgcggttttccctccc
caccaaattgttccactcccatttatcaattcaaagttgtttcatcccccttcctatttccccctcaatggcctgatgaacaatagacccttctcttgtg
ggaaatccagtcggattactggtgtaccccccccccaacccttggctctttgaaaatcataaccccttcgcaatagagataagagacccgcttataaata
tagaatattacctctcccattgtcacccacccccacctcttgtttttgttaaactctaatatntccacgaataatgatgtaaaaaacatcgcgcgggcgt
ggatgtacatgtttttatctccccacccatataaattcttctccgcgcgccnntttatgttccaactcatctgttttcacacatattataatacactctt
gtcgaaaaatgagcgttctgcgtcttactaccacttgactgtaaatatacgccgcgtacgtaacagctgttggtaacagtgttgtaaagtcccctacacc
acgcttctccacttttatctccgcactctccacgcgccc
3' EST: CF783421
[+]tttttttttttttttttttttttgaggattgcaaaacattttattatgttctcccttcacagtaaacagaatttcagtgctgaatgaccatagaaaacta
aatctacatgtcacatattgttattacctatcaagatgtaaaaaaaaacacaataaatgtacatgtgaataatgatacatataacatagcagcagtttgt
gccaattacatcatggatttaggtatttgcaaagattttggaatgagccatattttattaaaaagcaattagataaagacaatgcaggttgcaccaactc
cccccccccccttatactacaggtattcaataagctttccactcgagtagttgttataaagtaacaaagctactgaaaacatgcactgttggagggttac
ggcaggcatctcaacattgatatatatctaccctcttctcttttaaattagattgtaaaacagaagagtttgttttaaattaatgcttgaaaaagcgaag
aattgatagctaaaatgtaattagggaggtaaactatatctccatgatgtcacttacatattagtctctgtaggtcttcattgtaccttatggtaaacat
tatttttgttttatactgngattgctgcactgatagaaaaacttgcaccatacagtcacaagcttagaagcatttacggtggacaatgggaaagtcccag
cttcatcgtattcttgcttgggtgatccacattttnctggaagggttgaaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: