XB-CLONE-4353737
Clone Name: IMAGE:7026285
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Gene:
mrpl53
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077056
[+]
GATTGGCTGCATGTGTGCGGGATGATGGCTGCTGTCGGTGCTTCGGAGCTGCTGCTAAAGTCAGTGAAGTCCATCGCTGTGCGATTCTGTCCCTTTGAGC
CCAATGTCCGGCACGTTAGAGAATTTTTGGATATTATAAACTCCAAAAAAATCCGCTCTTCCAACGCAAACTGTGACATTGCTGTAGATGTGAGGCATGA
TCGGAGTGAACCCCACGTGGATATTCTGTTTGCTGACGGTGAGCGACTTGTGATAAAAGGACCCAATGTGACCTCCAAGGAAATGTTATCAGCTTTAAAT
GTAAAATGTGCCTCCAAGAACCTGCAAGTAAAAGACAGTGGAAAAAAGTAGTGTGTGGAGAGGCTTGCCTCTTGTTCATCATTCCTGAGCCATTCCATTA
AGCAACCTTCCACCTGGGGCACAGCAGCCGCACAATCTCTTTCTGCTATGACTGGCAGGGAACTTGCCTTGATAAGCCCAAGCCGATGGCTGTCTTCTGG
TGACCCGGCAGAGTCACTCTGAGCTGAAATCCATAATTCACACAAGATCCTCGGATTTGCTTTCTCTTTGTTAATATTTTTTTTTCAGCAGCATAAAAAA
ACGTATACAGAAACACATTTCCACTGTTATAGAGCTAAATATAACTTATCTGAAACCAGAAAAAAAAAAAAAAAA
5' EST: CF783423
[+]taattaatcccccccccccccgattggctgcatgtgtgcgggatgatggctgctgtcggtgcttcggagctgctgctaaagtcagtgaagtccatcgctg
tgcgattctgtccctttgagcccaatgtccggcacgttagagaatttttggatattataaactccaaaaaaatccgctcttccaacgcaaactgtgacat
tgctgtagatgtgaggcatgatcggagtgaaccccacgtggatattctgtttgctgacggtgagcgacttgtgataaaaggacccaatgtgacctccaag
gaaatgttatcagctttaaatgtaaaatgtgcctccaagaacctgcaagtaaaagacagtggaaaaaagtagtgtgtggagaggcttgcctcttgttcat
cattcctgagccattccattaagcaaccttccacctggggcacagcagccgcacaatctctttctgctatgactggcagggaacttgccttgataagccc
aagccgatggctgtcttctggtgaaccggcagagtcactctgaactgaaatccataattcacacaaaaatcctcggaattgcttttcccctttgttaata
ttttattttttcagccagccttaaaaaaaacggtattacggaaaacaccattttccactggtttattagaagcttaaataataaactttaatctgtaaac
cccggaaaaaaaatacatagataaattggggtcccaccttagtttctaaaaaagtgagatcccccaacccgccggagggaaatctttggtggcccgggga
cccctcaaaatatttgtgtttaattgggccaaccctttattaaaagggggttttaccaaaaaattaaaaagcctatttaaaacggcttcctcccagaaat
3' EST: CF783424
[+]tttttttttttttttctggtttcagataagttatatttagctctataacagtggaaatgtgtttctgtatacgtttttttatgctgctgaaaaaaaaata
ttaacaaagagaaagcaaatccgaggatcttgtgtgaattatggatttcagctcagagtgactctgccgggtcaccagaagacagccatcggcttgggct
tatcaaggcaagttccctgccagtcatagcagaaagagattgtgcggctgctgtgccccaggtggaaggttgcttaatggaatggctcaggaatgatgaa
caagaggcaagcctctccacacactacttttttccactgtcttttacttgcaggttcttggaggcacattttacatttaaagctgataacatttccttgg
aggtcacattgggtccttttatcacaagtcgctcaccgtcagcaaacagaatatccacgtggggttcactccgatcatgcctcacatctacagcaatgtc
acagtttgcgttggaagagcggatttttttggagtttataatatccaaaaattctctaacgtgccggacattgggctcaaagggacagaatcgcacagcg
atggacttcactgactttagcagcagctccgaagcaacgacagcagccatcatcccgcacacatgcagccaatcggggggggggggggattaattaaccc
cgggtgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: