XB-CLONE-4353734
Clone Name: IMAGE:7026284
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783426 [+]
taattaatcccccccccccccctttcctctgtggctcggacgcgagtgctttatggaggtgtcgtgtatcttttacaagctgctacgttgaattatggca
tcagttccttcttcactggggcttctctctggatatatttcctctgggtcagatgacgaggaagaaacagtgcagaaagcaatttgtactcctacttcat
cagtaaattgctttaaaaatcctcactccagaagcgaagagggacctgttgcaaaaaaaggtgaaactgaaaaagaatgtatgcatgttaatatctctga
ttcatctgtccgcctcccagccccgtgtcttgggatcccaaaacaccagtggctttggtggggtgttttctaacccatttcacgaggaggaacaagcaca
gatgagcactactggagaggcatgttaagctaaatgacaacacctgggcactaagcaaaagtgcctgcttaacttacccaaaaggaggggcgctgccgtt
atggaacgagatgccaaataccaccctgggtactgactttacccccagggaaatggccaggagcagttggtacaaaaacctggggtaacaaacttatttc
ttttaaatggaaacattccgggtggcccatttctggaagggagggaaataaaaaacaaaaagggggatccccctattttccaaaaagggccccgcccccc
ccccgcttgtaacccttcgcccgttgggccccaataattccattaatcttgccatccttaaaaaggggggcaacccaaattatacccccccattccattt
tccagatttttcccccacacaagagcattttttttctccccccgccccctcccaatttttttggcttctctggccaaattctttatccccatgggcacct
cctatatcctt
3' EST: CF783425 [+]ttttttttttttttcctccatcagaatggcacccgatgtttcatttaataaatagtttgtacccagctcttgtaccactgctcttgcatttccctggggt
aagtcgctaccatggctgtatttgcatcttgttccataacggcaccgcccatccttttggtaagaataagcaggcacctctgcttagtgcccagttgttg
tcacttagctttacatgcctctccagtatgctcatctgtgcttgttcctcctcgtgaaatgggttagaaaacaccccaccaaagccactggtgttttggg
atcccaagacagggggctgggaggcggacagatgaatcagagatattaacatgcatacattctttttcagtttcacctttttttgcaacaggtcccctct
tcgcttctggagtgaggatttttaaagcaatttactgatgaagtaggagtacaaattgctttctgcactgtttcttcctcgtcatctgacccagaggaaa
tatatccagagagaagccccagtgaagaaggaactgatgccataattcaacgtagcagcttgtaaaagatacacgacacctccataaagcactcgcgtcc
gaccacagagaaggggggggggggggggggggggtaataattaaactt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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