XB-CLONE-4353729
Clone Name: IMAGE:7026281
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Gene:
tuba5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783428
[+]
taattaatccccccccccccccgagttgctgtgttggcggagacccgtagcgcgtctatagtcaacggagccaaagaggggcgagtgtaggaagactgaa
ggttatctggtcttttatttagttctaccggtgcgcacacatctaatcagaaaatgcgtgagtgtatatctgtccatgttggccaggctggagttcagat
cggtaatgcatgctgggaactttactgcctagaacatgggatccaaccagatggtcagatgcctagtgacaaaactattgggggtggagatgattccttt
aacactttctttagtgaaactggagcaggaaaacatgttcctagagcagtgtttgtagacctggaatctgcagtcatagatgaggttcgtaatgggacat
ataggcagctttttcaccctgagcagcttataactggtaaagaggatgctgccaataattatgcacgtgggcactacactgtaggaaaagagatcattga
cttggtgctggaaagagtacggaaactggctgaccaatgcacaggtctccagggttttctgattttccacagctttggaggaggaacaggatctggattt
acatccttgcttatggagagactatctgtagattatggcaagaagtccaagttgggaatttgccatctatccagcccccacaagtggtctacagctgttg
gtttgaacccttataatttccatttctttaccacaacaccacttacacctggggaggcacctcctggacctggtggccatttttattggggtgggagaat
aaatggaaggggcccaatttttatatggaacacataatgggccaggggcgcggc
3' EST: CF783429
[+]tttttttttttttttggaataaaaacctttatttcaagcagctatgtcaggaatgacattctgtataatgcacttcaatggaatggcactaatattcctc
cccttcatcctctccctcaatagagtctgtgcccacttcctcataatctttttctaaagcagcaagatcctctcgtgcctcagaaaattcaccctcctcc
atgccttcaccaacataccaatgcacaaaagcacgttttgcatacataaggtcaaacttatggtccagacgtgcccaggcttcggcaatagctgttgtgt
tgctgagcatacagactgcgcgctgaactttggcaagatcaccacctggaaccgcagtaggtggctggtaatttattcccaccttaaaacctgttgggca
ccagtccacaaactgtatagaacgtttggtcttaatagccgcaattgcagcattcacatcctttggcacaacatctccacgatataacatacagcaggcc
atgtacttgccgtgtcgtgggtcacattttaccatctggttagatggttcaaaacaggcatttgtgatttcagacacagagagctgctcatggtatgctt
tctcggcagaaattataggagagtacgtgacaagagggaagtgtatacgaggataaggcaccaaattggtctggaattctgtcaagtccacattgagggc
cccatcaaagcgcaaggaggctgtgatagaaaaactatctgggccatgaggcgggtcaaattaatgtaggttgggcgctcaatgtcgaagttccgcctgc
atatgtcataaatgggctca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: