XB-CLONE-4353726
Clone Name: IMAGE:7026280
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783431 [+]
taattaatcccccccccccccgtatatgctttaattgctgctgccagagctttgtccattttaattctacagtttgtaaatgaatgaaccaaattaaaag
tttgtaaatcaataaaacccaaatccttaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
aaaaaaaaaaaaaaaaaaaaaaaaaggggttcccttttttttaaagggccccccccccgggggaaattgggccgggccccactttttttttggcccctta
aaggggttaaaaaaaaaccaaaaccccccaaattttcaaaaaaaaacctttttttccccggctttcaagtgggggttggcccaaccccccaagggtttct
taacggggggggacgggaaaaaaattgggcccacccccggggccgaaaaaacccccaaaaaaaaaaattggtttaggtccttttggaggggaaaaaaacc
cccggggaaaggggtgccatttggggggggaaaaaccccagggctcccccccggggaaaaattttgaaagtttgccccttattatttcccccacccgggt
ggggaaaagcccccaggccccccccccggggggaaaaattttgccaaacctttgctttctcattttttttcacccacacattatccccccccccaaaata
ttccgcccactccccccgccccacaattttccccccaagtttctcccccctatttttctgcgcccccaggggggggaaacaaattttttgtttttttatt
ttatttacaaaaggggcgcgagagcgcccccccccgccgccctccttgaaagttttttctccacaaaaatatgtgggggaggggagggcttttttttttt
gagaagggaatacac
3' EST: CF783430 [+]tttttttttttttttctttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttaaa
gggggggggtttttttgttttccaaccttttaattggggccccccctttcccccccggaaaaaaaaaagggaaaaacccccggcccccccaaaaaaaaca
aaacggggggggggggggaaaaaaaacccccggggcaaaaaaaaaaaattccttttttcccccccccaaaaaaacccaggggccttgggccccggggggg
gccccccggggaaaaaaaggcaaagggggttttttcccaaggggccccccaaacccggggggggaaaaacaaagggggtggtttaaattgggcccccctt
caaaaaaacccaagggggggggcccaaaaaccccaagggggggatttccccccccctggggcgggggaaaccccgttccaagggcccctcccccccccca
aaaggggaaaccccccccgggcccccccgggggggcaaaaccccggggggggggccccccgggaaaatgggggttcccaaaaaaagccccccttaaaaaa
acccccccccgaaacccccccccccccctttgccccaagggggggggaaatttttccccccttttgaaaaaccccccccgatatttgggggccaaaaaaa
aacccccctatggacccaggggggggggggaaaaccttaaaaaccccccggggagaaaaaacccgtaacccccccccctattggttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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