XB-CLONE-4353720
Clone Name: IMAGE:7026278
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783435 [+]
taattaatcccccccccccccagccctaggaagcttttggtgctgcttcttgagcctagcatctgctaacgtaacaccggcatcaccatgactgacgcag
ccatctcttttgccaaggacttcttggccggcggtgtggccgcagctatctccaagaccgctgtagcacctattgaaagagtcaagcttctactgcaagt
ccaacatgcaagcaaacagatcaccgcagacaagcactacaagggcatcatggactgtgttgtcagaatccccaaaaaacaggggttcatgtcttttttg
gcggggtaaactttgccaacgtggattccgttaatttcccaaacccaggcccttcaactttccccttttcaaagaaaaattaccaagaaaaatccttccc
cgtggaaaaacgttaccaccaacgaataaccccccttttctggggggctcccttttttagctggggaaaacccttggtctttccccgggggggggggcat
tctccggggcgagaaatcccctctcccccccccccggccttttgtgggtttaacccccccctttggaaatattttcgtgccccgcgtgaccacacccctt
ctctttttcacccctttctcattatatgctggccgtgcacaataagaggcgcaacttattctcttgtctcaccaatattacacaggttgtttacaagagg
gggcgcactctcgggtaggataaacaaagccctctataatataataaacaaaacataatttcatcatcaatatctcttgtatgagtggggatttatataa
agggcgggttatttttagcctaccagcgagttatttttctccacccctctctttttttcccccccccgacaccgttgttacccccgctccattctcgcac
gccataccccaatacttgtccttcgctctatattggcctgaatacgtccncctcaatagatatatcacgctatacacctataatccgccc
3' EST: CF783434 [+]tttttttttttttttattttagatccgtattttttatttcctcaaacctaagttacttaaaaacatgaatgactgaaataaaaaataaaggagcgttgag
tcccactgaatcagcctgtcactgccaaagggccaaaaactgccaaggaggcacagttgcaggcactaaaagcagtaagagacttaaagggaatgattta
ttaatgtggaaagctggtgagatgtaatagaactgatcccaaaataccagccagtaaagattaccagctatattttataaataagggtgtgtttgacaaa
aaaaataaagaaggtccatgaatgttcaaggtatgttacataatacagcatgccaggtcacagacatctcaacaaggacaagtttagatgtatttcttca
gctcatcatacaacaccagcacaaaagcgccacccattcctctgagaacattggaccaggcacccttgaagaaagccctgctgccctcatcccgtgcaat
cttcctccagcagtcaattgttccactgtacatgatgtcagctcctttccttccagactgcatcatcatacgacgacgtactgtgtcaaatggataggag
acaaatccagctaccgctgttacagtctgagcaatcatccagctgatgataatgtgtgtggtcctggggatctggaagcataccttttagctgtatcata
gatgccaaaataagcagctctgtagatgatgatgccctgnaacagatacgttgaatccctggtacaagcccttgatcccatccgaatctgggagatcttt
aactaagcaatcaccaaagcccttga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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